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使用长读测序生成的高度连续基因组资源。

Highly Contiguous Genome Resource of Generated Using Long-Read Sequencing.

机构信息

State Key Laboratory of Crop Stress Biology in Arid Areas and College of Plant Protection, Northwest A&F University, Yangling, 712100, Shaanxi Province, China.

Department of Plant Pathology, University of Florida, Gainesville, FL, U.S.A.

出版信息

Mol Plant Microbe Interact. 2020 Jun;33(6):790-793. doi: 10.1094/MPMI-11-19-0316-A. Epub 2020 Apr 29.

DOI:10.1094/MPMI-11-19-0316-A
PMID:32163336
Abstract

is a plant-pathogenic fungus with a broad host range. It causes significant losses to important crops, including apple, pear, strawberry, and other Rosaceae and non-Rosaceae species. To date, two short read-based genomes are publicly available, but both are fragmented. In this study, we re-sequenced the genome of using nanopore long-read technology and refined the assembly with Hi-C map data. The resulting high-quality assembly is an important resource for further comparative and experimental studies with .

摘要

是一种具有广泛宿主范围的植物病原真菌。它会给包括苹果、梨、草莓和其他蔷薇科及非蔷薇科物种在内的重要作物造成重大损失。迄今为止,有两个基于短读长序列的基因组公开可用,但它们均是不完整的。在本研究中,我们使用纳米孔长读长技术重新测序了 的基因组,并使用 Hi-C 图谱数据来完善组装。由此得到的高质量组装是进一步与 进行比较和实验研究的重要资源。

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