Kanamatsu T, Hirano S
Department of Physiology, Toho University School of Medicine, Tokyo, Japan.
Neurochem Res. 1988 Oct;13(10):983-8. doi: 10.1007/BF00970772.
In an attempt to elucidate the relationship between endogenous methionine-enkephalin (ME) and vasoactive intestinal polypeptide (VIP) with generalized seizures, we determined regional brain levels of ME-like and VIP-like immunoreactivity (ME-LI and VIP-LI) in El mice during and after seizures induced by repeated tossing stimulation. The levels of ME-LI in the striatum and hippocampus of seizure-naive El mice (El-) were lower than those of the control ddY mice, the mother strain of El mice. Conversely, the level of VIP-LI in the medulla oblongata and pons of El- was higher than that of ddY mice. The level of ME-LI in the striatum of seizure-experienced El mice (El+) killed 96 hours after three consecutive seizures was high, while levels of VIP-LI in the striatum and hypothalamus were low, in comparison to those of El- mice. A detailed time-course study revealed that seizures in El mice caused (1) significant decreases in levels of ME-LI in the striatum and hippocampus during seizures, (2) a significant decrease of VIP-LI content in the striatum 3 hours after seizures, and (3) a significant increase in hypothalamic VIP-LI 9 hours after seizures. These observations suggest that ME and VIP may play some role in El mouse seizures.
为了阐明内源性甲硫氨酸脑啡肽（ME）和血管活性肠肽（VIP）与全身性癫痫发作之间的关系，我们测定了反复抛掷刺激诱发癫痫发作期间及发作后El小鼠脑内ME样和VIP样免疫反应性（ME-LI和VIP-LI）的区域水平。未发作过癫痫的El小鼠（El-）纹状体和海马中的ME-LI水平低于其母系对照ddY小鼠。相反，El-小鼠延髓和脑桥中的VIP-LI水平高于ddY小鼠。与El-小鼠相比，连续三次癫痫发作后96小时处死的有癫痫发作经历的El小鼠（El+）纹状体中的ME-LI水平较高，而纹状体和下丘脑中的VIP-LI水平较低。一项详细的时间进程研究表明，El小鼠的癫痫发作导致：（1）癫痫发作期间纹状体和海马中ME-LI水平显著降低；（2）癫痫发作后3小时纹状体中VIP-LI含量显著降低；（3）癫痫发作后9小时下丘脑VIP-LI显著增加。这些观察结果表明，ME和VIP可能在El小鼠癫痫发作中发挥一定作用。
