Shenzhen Second People's Hospital, First Affiliated Hospital of Shenzhen University, College of Life Sciences and Oceanography Shenzhen University, Shenzhen, Guangdong, 518035, China.
Shenzhen Immunotherapy Biotechnology Co., Ltd, Shenzhen, Guangdong, 518109, China.
Sci Rep. 2020 Mar 13;10(1):4700. doi: 10.1038/s41598-020-61455-8.
Allergic responses are mainly caused by IgE, which is often located on the cell surface. The current diagnostic method detects both allergen-specific IgE and total IgE levels, but a number of allergic patients have a normal serum IgE level, which is a poor clinical correlate for allergy. Here, we developed a simple method to detect the level of cell-bound IgE by dissociating it from blood cells with lactic acid. Dissociated cell-bound IgE and plasma IgE levels were detected using the same ELISA kit at the same time. We established two clinical cohorts: an allergic patient group and a healthy participant group. In general, cell-bound IgE correlated well with plasma IgE; however, some patients exhibited high cell-bound IgE levels but low plasma IgE levels. We recommended 350 ng/mL peripheral blood total IgE (cell-bound IgE + plasma IgE) as the cut-off value for allergy diagnosis. Using this indicator, 90.32% of our allergic patients were correctly diagnosed. The peripheral blood total IgE level is a promising clinical diagnostic indicator in allergic patients and will provide more guidance for allergy diagnosis and therapeutic evaluation.
过敏反应主要由 IgE 引起，IgE 通常位于细胞表面。目前的诊断方法同时检测过敏原特异性 IgE 和总 IgE 水平，但许多过敏患者的血清 IgE 水平正常，这与过敏的临床相关性较差。在这里，我们开发了一种简单的方法，通过用乳酸从血细胞中解离来检测细胞结合 IgE 的水平。同时使用相同的 ELISA 试剂盒检测解离的细胞结合 IgE 和血浆 IgE 水平。我们建立了两个临床队列：过敏患者组和健康参与者组。一般来说，细胞结合 IgE 与血浆 IgE 相关性良好；然而，一些患者表现出高细胞结合 IgE 水平但低血浆 IgE 水平。我们建议将外周血总 IgE（细胞结合 IgE+血浆 IgE）350ng/mL 作为过敏诊断的截止值。使用该指标，90.32%的过敏患者得到了正确诊断。外周血总 IgE 水平是过敏患者有前途的临床诊断指标，将为过敏诊断和治疗评估提供更多指导。
