Jiangsu Key Laboratory of Advanced Catalytic Materials and Technology, School of Petrochemical Engineering, Changzhou University, Changzhou, 213164, China.
Advanced Catalysis and Green Manufacturing Collaborative Innovation Center, Changzhou University, Changzhou, 213164, China.
Mikrochim Acta. 2020 Mar 13;187(4):227. doi: 10.1007/s00604-020-4210-2.
Ultrasensitive and selective determination of bisphenol A (BPA) was achieved by a solid-state electrochemiluminescence (ECL) aptasensor. The Ti-based MOFs (MIL-125, MIL stands for Materials of Institute Lavoisier) have porous structure and large specific surface area. They were used as a carrier for Ru(bpy) by a hydrothermal synthesis method. The Ru(bpy) encapsulated MIL-125 (Ru(bpy)@MIL-125) was dropped on a glass carbon electrode (GCE) and used as working electrode. After that, the thiol-based aptamer of BPA was used to modify the working electrode by Ti-S bond. As a result of the specific binding affinity between aptamer and BPA, the ECL signal was significantly quenched. Thus, a selective electrochemiluminescence (ECL) aptasensor for BPA was developed. The ECL aptasensor based on Ru(bpy)@MIL-125 has a good ECL response toward detecting BPA. Under optimal conditions, the aptasensor can detect BPA in a wide linear range from 1.0 × 10 to 1.0 × 10 M with an excellent determination limit of 6.1 × 10 M (S/N = 3). The ECL aptasensor can selectively detect BPA in a mixture containing BPA interference compounds.
基于固态电化学发光（ECL）适体传感器实现了对双酚 A（BPA）的超灵敏和选择性测定。Ti 基 MOFs（MIL-125，MIL 代表 Lavoisier 研究所的材料）具有多孔结构和大的比表面积。它们通过水热合成方法被用作 Ru(bpy)的载体。Ru(bpy)封装的 MIL-125（Ru(bpy)@MIL-125）被滴在玻碳电极（GCE）上，并用作工作电极。之后，BPA 的基于硫醇的适体通过 Ti-S 键被用于修饰工作电极。由于适体与 BPA 之间的特异性结合亲和力，ECL 信号被显著猝灭。因此，开发了一种用于 BPA 的选择性电化学发光（ECL）适体传感器。基于 Ru(bpy)@MIL-125 的 ECL 适体传感器对 BPA 具有良好的 ECL 响应。在最佳条件下，该适体传感器可以在 1.0×10 到 1.0×10 M 的宽线性范围内检测 BPA，具有优异的检测限为 6.1×10 M（S/N=3）。ECL 适体传感器可以在含有 BPA 干扰化合物的混合物中选择性地检测 BPA。
