Protein Research Center, Shahid Beheshti University, Tehran, Iran.
J Biomed Mater Res B Appl Biomater. 2020 Aug;108(6):2633-2642. doi: 10.1002/jbm.b.34595. Epub 2020 Mar 14.
Separation of tumor cells is a promising approach that helps not only in early detection of cancer but also as an efficient tool that holds great importance in prohibiting cancer cell mutation, drug resistance to treatments, and in granting successful adjuvant therapies. As one of the highly efficient processes for the separation of single cells, tumor cells, and specific proteins from fresh whole blood, a magnetic iron oxide nanoparticle (IONP)-based immunomagnetic separation technique has been developed in this article. The synthesized IONPs were modified with antibodies (Abs) against human epithelial growth factor receptor 2 (HER2), which is overexpressed and/or amplified in about 15% of breast cancer patients with several types of human cancer cells. The prepared Ab-conjugated IONPs (Ab-IONPs) attach HER2-positive cancer cells exclusively and can serve as specific high-efficient single-cell separation agents. The results showed that the magnetic IONPs have been successfully attached to the Abs via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide/N-hydroxysuccinimide linkers. Maximum targeting efficiency of the Ab-IONP complex, which was 94.5 ± 0.8% for BT474 and 70.6 ± 0.4% for mixture of cells (BT474 and MCF7), was achieved with a minimum amount of Abs, to provide an economically efficient single-cell detection device.
肿瘤细胞分离是一种很有前途的方法，不仅有助于癌症的早期检测，而且作为一种有效的工具，在抑制癌细胞突变、对治疗的耐药性以及成功辅助治疗方面具有重要意义。本文开发了一种基于磁性氧化铁纳米颗粒（IONP）的免疫磁分离技术，用于从新鲜全血中分离单个细胞、肿瘤细胞和特定蛋白质。合成的 IONP 用针对人表皮生长因子受体 2（HER2）的抗体（Abs）进行了修饰，HER2 在约 15%的乳腺癌患者中过度表达和/或扩增，并且在几种类型的人类癌细胞中也存在。制备的 Ab 缀合的 IONP（Ab-IONP）专门附着 HER2 阳性癌细胞，并可用作特定的高效单细胞分离剂。结果表明，通过 1-乙基-3-（3-二甲基氨基丙基）碳二亚胺/N-羟基琥珀酰亚胺接头，成功地将磁性 IONP 附着到 Abs 上。Ab-IONP 复合物的最大靶向效率为 94.5±0.8%（用于 BT474）和 70.6±0.4%（用于 BT474 和 MCF7 混合物），使用最少的 Abs 实现，从而提供了一种经济高效的单细胞检测装置。
