State Key Laboratory of Food Science and Technology, Nanchang University, Nanchang 330047, PR China.
Biomaterials. 2011 Dec;32(36):9758-65. doi: 10.1016/j.biomaterials.2011.08.076. Epub 2011 Sep 14.
A highly efficient process using iron oxide magnetic nanoparticles (IO)-based immunomagnetic separation of tumor cells from fresh whole blood has been developed. The process involved polymer coated 30 nm IO that was modified with antibodies (Ab) against human epithelial growth factor receptor 2 (anti-HER2 or anti-HER2/neu) forming IO-Ab. HER2 is a cell membrane protein that is overexpressed in several types of human cancer cells. Using a HER2/neu overexpressing human breast cancer cell line, SK-BR3, as a model cell, the IO-Ab was used to separate 73.6% (with a maximum capture of 84%) of SK-BR3 cells that were spiked in 1 mL of fresh human whole blood. The IO-Ab preferentially bound to SK-BR3 cells over normal cells found in blood due to the high level of HER2/neu receptor on the cancer cells unlike the normal cell surfaces. The results showed that the nanosized magnetic nanoparticles exhibited an enrichment factor (cancer cells over normal cells) of 1:10,000,000 in a magnetic field (with gradient of 100 T/m) through the binding of IO-Ab on the cell surface that resulted in the preferential capture of the cancer cells. This research holds promise for efficient separation of circulating cancer cells in fresh whole blood.
已经开发出一种使用基于氧化铁纳米磁珠（IO）的免疫磁分离从新鲜全血中分离肿瘤细胞的高效方法。该过程涉及聚合物包覆的 30nm IO，其被针对人表皮生长因子受体 2（抗 HER2 或抗 HER2/neu）的抗体（Ab）修饰，形成 IO-Ab。HER2 是一种在多种人类癌细胞中过度表达的细胞膜蛋白。使用过表达 HER2/neu 的人乳腺癌细胞系 SK-BR3 作为模型细胞，IO-Ab 用于分离 1mL 新鲜人全血中掺入的 73.6%（最大捕获率为 84%）的 SK-BR3 细胞。由于癌细胞表面的 HER2/neu 受体水平较高，与正常细胞表面不同，IO-Ab 优先与癌细胞结合，而不是与血液中的正常细胞结合。结果表明，在磁场（梯度为 100T/m）中，纳米级磁纳米颗粒通过 IO-Ab 在细胞表面的结合，表现出 1:1000000 的富集因子（癌细胞与正常细胞的比值），从而优先捕获癌细胞。这项研究有望高效分离新鲜全血中的循环癌细胞。
