Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford St., Cambridge, MA 02138, United States.
Department of Chemistry and Chemical Biology, Harvard University, 12 Oxford St., Cambridge, MA 02138, United States.
Curr Opin Biotechnol. 2020 Oct;65:94-101. doi: 10.1016/j.copbio.2020.02.003. Epub 2020 Apr 1.
Radical enzymes catalyze some of the most chemically challenging CC bond-forming and bond-breaking reactions. Advances in DNA sequencing have accelerated the discovery of radical enzymes from microbes, including radical S-adenosylmethionine (rSAM) enzymes, glycyl radical enzymes (GREs), and diiron enzymes. These enzymes catalyze various reactions that yield products of industrial relevance (e.g. aromatics, hydrocarbons, and natural product derivatives), making their incorporation into engineered metabolic pathways enticing. Elucidating the mechanisms of radical enzymes that cleave and construct CC bonds will enable further enzyme discovery and engineering efforts.
自由基酶催化一些最具挑战性的碳-碳键形成和断裂反应。DNA 测序技术的进步加速了微生物中自由基酶的发现，包括自由基 S-腺苷甲硫氨酸（rSAM）酶、甘氨酰基自由基酶（GREs）和二铁酶。这些酶催化各种反应，生成具有工业相关性的产物（例如芳烃、碳氢化合物和天然产物衍生物），因此将它们纳入工程代谢途径具有吸引力。阐明断裂和构建碳-碳键的自由基酶的机制将能够进一步促进酶的发现和工程化努力。
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