Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education and Yunnan Province, School of Chemical Science and Technology, Yunnan University, Kunming 650091, PR China; State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, PR China.
Lanzhou University of Technolog, Lanzhou 730050, PR China.
Fitoterapia. 2020 Jun;143:104547. doi: 10.1016/j.fitote.2020.104547. Epub 2020 Mar 12.
Five new indole alkaloids, kopsiofficines H-L (1-5), along with fourteen known alkaloids (6-19) were isolated from the stems of Kopsia officinalis. Their structures were elucidated by extensive NMR, mass spectroscopic analyses and comparison to the reported data. All the isolated compounds were evaluated their anti-inflammatory activities by inhibiting IL-1β, PGE2 and TNF-α secretion in lipopolysaccharide (LPS)-activated RAW264.7 cells. Compounds 2, 3, 6, 7, 11, 12, 15, and 17 show significant anti-inflammatory activities. These results demonstrate pharmacodynamic substance basis of these folkloric claims.
从黄桐的茎中分离得到了五个新的吲哚生物碱，即 kopsiofficines H-L（1-5），以及 14 个已知的生物碱（6-19）。通过广泛的 NMR、质谱分析和与已报道的数据进行比较，阐明了它们的结构。所有分离得到的化合物都通过抑制脂多糖（LPS）激活的 RAW264.7 细胞中 IL-1β、PGE2 和 TNF-α 的分泌来评估其抗炎活性。化合物 2、3、6、7、11、12、15 和 17 表现出显著的抗炎活性。这些结果证明了这些民间传说的药效物质基础。
