Jiangsu Key Laboratory of Bioactive Natural Product Research and State Key Laboratory of Natural Medicines, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China.
Jiangsu Key Laboratory of Bioactive Natural Product Research and State Key Laboratory of Natural Medicines, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, People's Republic of China.
Fitoterapia. 2020 Jun;143:104546. doi: 10.1016/j.fitote.2020.104546. Epub 2020 Mar 12.
Eight new compounds (Entanutilins O-V; 1-8), including four limonoids, two steroids, one triterpenoid and one lignan were isolated from the stem barks of Entandrophragma utile. Their structures were determined by detailed spectroscopic analyses (HRESIMS and 1D/2D-NMR). Bioactivity screening indicated that compounds 1, 6 and 7 exhibited effective in reversing resistance in MCF-7/DOX cells at a nontoxic concentration of 30 μM with 18.18-, 7.43- and 7.94-fold enhancing effect, respectively, meanwhile, compounds 5 and 6 showed moderate NO inhibitory activities in LPS-activated RAW 264.7 macrophages.
从非洲桃花心木的茎皮中分离得到了 8 种新化合物（Entanutilins O-V；1-8），包括 4 种柠檬苦素、2 种甾体、1 种三萜和 1 种木脂素。通过详细的光谱分析（HRESIMS 和 1D/2D-NMR）确定了它们的结构。生物活性筛选表明，化合物 1、6 和 7 在非毒性浓度 30μM 时有效逆转 MCF-7/DOX 细胞的耐药性，增强作用分别为 18.18、7.43 和 7.94 倍，同时，化合物 5 和 6 在 LPS 激活的 RAW 264.7 巨噬细胞中显示出中等的 NO 抑制活性。
