Department of Engineering for Innovation, University of Salento, Lecce, Italy; Typeone Srl, Campus Ecotekne, Via per Monteroni, Lecce, Italy.
Department of Engineering for Innovation, University of Salento, Lecce, Italy.
Int J Biol Macromol. 2020 Jul 1;154:291-306. doi: 10.1016/j.ijbiomac.2020.03.082. Epub 2020 Mar 13.
Type I collagen is the most abundant protein of the human body. Due to its favourable properties, collagen extracted from animal tissues is adopted to manufacture a wide range of devices for biomedical applications. Compared to bovine and porcine collagens, which are the most largely used, equine collagen is free from the risk of zoonosis, has no reported immune reactions, and has not religious constraints. In this work, a recently available type I collagen extracted from horse tendon was evaluated and compared with a commercially available collagen isoform derived from the same species and tissue. Detailed physical, chemical and biological investigations were performed, in agreement with the requirements of the current standard for the characterization of type I collagen to be used for the manufacture of Tissue Engineering Medical Products. To the best of our knowledge, this is the first report on the complete primary structure of the investigated collagen.
I 型胶原蛋白是人体中最丰富的蛋白质。由于其良好的特性，从动物组织中提取的胶原蛋白被用于制造各种用于生物医学应用的设备。与最广泛使用的牛胶原和猪胶原相比，马胶原不存在人畜共患病的风险，据报道也没有免疫反应，并且没有宗教限制。在这项工作中，评估了一种最近从马肌腱中提取的可用的 I 型胶原蛋白，并将其与一种市售的、源自同一物种和组织的胶原蛋白同工型进行了比较。根据当前用于制造组织工程医疗产品的 I 型胶原蛋白特性描述的标准要求，进行了详细的物理、化学和生物学研究。据我们所知，这是首次对所研究的胶原蛋白的完整一级结构进行的报告。
