URT "Genomics of Diabetes," Institute of Experimental Endocrinology and Oncology, National Research Council, Naples, Italy.
Department of Translational Medicine, "Federico II" University of Naples, Naples, Italy.
J Tissue Eng Regen Med. 2020 May;14(5):701-713. doi: 10.1002/term.3032. Epub 2020 Apr 8.
Diabetic patients display increased risk of periodontitis and failure in bone augmentation procedures. Mesenchymal stem cells (MSCs) and platelet-rich plasma (PRP) represent a relevant advantage in tissue repair process and regenerative medicine. We isolated MSCs from Bichat's buccal fat pad (BFP) and measured the effects of glucose and PRP on cell number and osteogenic differentiation potential. Cells were cultured in the presence of 5.5-mM glucose (low glucose [LG]) or 25-mM glucose (high glucose [HG]). BFP-MSC number was significantly lower when cells were cultured in HG compared with those in LG. Following osteogenic differentiation procedures, calcium accumulation, alkaline phosphatase activity, and expression of osteogenic markers were significantly lower in HG compared with LG. Exposure of BFP-MSC to PRP significantly increased cell number and osteogenic differentiation potential, reaching comparable levels in LG and in HG. Thus, high-glucose concentrations impair BFP-MSC growth and osteogenic differentiation. However, these detrimental effects are largely counteracted by PRP.
糖尿病患者牙周炎和骨增量手术失败的风险增加。间充质干细胞（MSCs）和富含血小板的血浆（PRP）在组织修复过程和再生医学中具有重要优势。我们从比夏氏颊脂垫（BFP）中分离出 MSCs，并测量了葡萄糖和 PRP 对细胞数量和成骨分化潜能的影响。细胞在 5.5-mM 葡萄糖（低葡萄糖[LG]）或 25-mM 葡萄糖（高葡萄糖[HG]）存在的条件下培养。与 LG 相比，HG 中细胞培养时 BFP-MSC 的数量明显较低。成骨分化后，与 LG 相比，HG 中钙积累、碱性磷酸酶活性和成骨标志物的表达明显较低。PRP 暴露于 BFP-MSC 可显著增加细胞数量和成骨分化潜能，达到 LG 和 HG 中的可比水平。因此，高葡萄糖浓度会损害 BFP-MSC 的生长和成骨分化。然而，PRP 很大程度上抵消了这些不利影响。
