Department of Oral Medicine and Radiology, Sree Balaji Dental College and Hospital, BIHER University, Chennai, Tamil Nadu, India.
Department of Human Genetics Research Laboratory, Sree Balaji Dental College and Hospital, BIHER University, Chennai, Tamil Nadu, India.
J Indian Soc Pedod Prev Dent. 2020 Jan-Mar;38(1):8-13. doi: 10.4103/JISPPD.JISPPD_222_19.
Though factors such as diet, oral hygiene, salivary flow and surface characteristics of the tooth enamel play a role in the causation of dental caries, genetic factors also contribute significantly in influencing the susceptibility or resistanance to the occurrence of caries.
To analyse the relationship between a single nucleotide polymorphism in the vitamin D receptor gene TaqI locus and associate its presence with dental caries in children.
A case control study was conducted among 120 children in the age group of 6-12 years. 60 cases with a DMFT of >3 and 60 controls with a DMFT of 0 were selected. Genomic DNA was extracted from salivary samples collected from the patients and presence of polymorphism was analyzed by PCR-RFLP.
Pearson chi square test and Fisher exact tests were used for comparison of differences in genotypes or allele frequencies between groups. No significant difference between the cases and control was observed among the different genotypes and the alleles. However, there was an inclination in the incidence of caries with the genotype 'tt' and 'Tt' when compared with the genotype'TT'.
In this study done to analyse the SNP in vitamin D receptor gene and its association with dental caries in children indicates a higher caries risk for a patient with't' allele and 'tt' genotype.
尽管饮食、口腔卫生、唾液流量和牙釉质表面特性等因素在龋齿的发生中起作用，但遗传因素也在很大程度上影响着龋齿的易感性或抵抗力。
分析维生素 D 受体基因 TaqI 基因座中单核苷酸多态性与儿童龋齿之间的关系，并分析其与龋齿的相关性。
对 120 名年龄在 6-12 岁的儿童进行病例对照研究。选择 DMFT>3 的 60 例病例和 DMFT 为 0 的 60 例对照。从患者的唾液样本中提取基因组 DNA，并通过 PCR-RFLP 分析多态性的存在。
Pearson 卡方检验和 Fisher 确切检验用于比较组间基因型或等位基因频率的差异。不同基因型和等位基因之间在病例和对照组之间没有观察到显著差异。然而，与 TT 基因型相比，基因型“tt”和“Tt”的龋齿发生率有一定的倾向。
本研究分析了维生素 D 受体基因中的 SNP 及其与儿童龋齿的关系，表明携带“t”等位基因和“tt”基因型的患者患龋齿的风险更高。
