Hu X P, Li Z Q, Zhou J Y, Yu Z H, Zhang J M, Guo M L
School of Stomatology, Northwest University for Nationalities, Lanzhou, Gansu, China.
School of Stomatology, LanZhou University, Lanzhou, Gansu, China.
Genet Mol Res. 2015 Sep 28;14(3):11631-8. doi: 10.4238/2015.September.28.15.
Environmental influences on the development and progression of dental caries are well known; however, there is little evidence of a genetic component imparting susceptibility to dental caries. The aim of this study was to investigate the relationship between a single nucleotide polymorphism in the vitamin D receptor TaqI locus and dental caries susceptibility in a Chinese population. This case-control study was conducted with a case group (264 patients with dental caries from northwestern China) and a control group (219 individuals without dental caries or systemic disease from the same area). DNA was extracted from the peripheral venous blood of the study participants; the distribution of TaqI locus genotypes and allele frequencies was determined via polymerase chain reaction-restriction fragment length polymorphism. The data obtained were statistically analyzed using the Hardy-Weinberg equilibrium and Chi-square test. The frequency of the Tt genotype in the case group (14.0%) was significantly higher than that in the control group (4.3%), as determined using the genotype TT as the reference. The risk of dental caries was increased 3.8-fold in individuals with the heterozygous Tt genotype compared to that in the individuals with the TT genotype. The proportion of the 't' allele in the case group (7.0%) and the control group (2.1%) was observed to be significantly different [P = 0.0003; OR = 3.592, confidence interval 95% (1.790-7.208)]. Our results therefore suggested that the allele 't' might be a genetic factor determining dental caries susceptibility in individuals from the northwest of China.
环境对龋齿发生和发展的影响是众所周知的；然而，几乎没有证据表明存在赋予龋齿易感性的遗传因素。本研究的目的是调查维生素D受体TaqI位点的单核苷酸多态性与中国人群龋齿易感性之间的关系。本病例对照研究包括病例组（来自中国西北部的264例龋齿患者）和对照组（来自同一地区的219例无龋齿或全身性疾病的个体）。从研究参与者的外周静脉血中提取DNA；通过聚合酶链反应-限制性片段长度多态性确定TaqI位点基因型和等位基因频率的分布。使用哈迪-温伯格平衡和卡方检验对获得的数据进行统计学分析。以基因型TT作为参照，病例组中Tt基因型的频率（14.0%）显著高于对照组（4.3%）。与TT基因型个体相比，杂合子Tt基因型个体患龋齿的风险增加了3.8倍。观察到病例组（7.0%）和对照组（2.1%）中“t”等位基因的比例存在显著差异[P = 0.0003；OR = 3.592，95%置信区间（1.790 - 7.208）]。因此，我们的结果表明，“t”等位基因可能是决定中国西北部个体龋齿易感性的一个遗传因素。
