Pereira Viviane, de Barros Neuza Biguinati, Macedo Sharon Rose Aragão, Dos Santos Ferreira Amália, Kanis Luiz Alberto, Nicolete Roberto
1Laboratório de Biotecnologia Aplicada à Saúde, Fundação Oswaldo Cruz - Rondônia, Porto Velho, RO Brazil.
Instituto de Ensino Superior de Rondônia - IESUR, Ariquemes, RO Brazil.
J Parasit Dis. 2020 Mar;44(1):79-87. doi: 10.1007/s12639-019-01162-y. Epub 2019 Sep 20.
Cutaneous leishmaniasis (CL), a clinical condition caused mainly by in Brazil, is characterized by topical, painless ulcers. The current treatment, based on intravenous administration of pentavalent antimonials, presents low adherence by patients and may cause serious adverse effects, leading to the need for searching new therapeutic options. Thus, this study aimed at evaluating a topical administration of "intelligent dressings" as an alternative treatment for CL. BALB/c mice were infected with promastigotes. Afterward, lesions were treated with hydrophobic dressings incorporated with clinically used drugs. After lesion development, the following analyses were carried out: measurement of lesion diameters, biochemical analyses of serum, evaluation of the recovery of amastigote forms and histological analyses. No significant clinical changes in serum parameters were observed. The group that was treated with dressings impregnated with Glucantime displayed the lowest number of amastigotes recovered from tissues (parasite load). Conventional treatment with Glucantime (i.p.) was also able to reduce parasite load. After 6 weeks from the measurement of the lesions mice treated with dressings impregnated with Pentamidine displayed the smallest values. Representative histological aspects of the lesions showed the absence or few amastigotes inside the macrophages when mice were treated with dressings impregnated with Glucantime and Pentamidine, respectively. The findings presented here indicate that the topical treatments may constitute an alternative treatment option for CL.
皮肤利什曼病（CL）是一种主要由 在巴西引起的临床病症，其特征为局部无痛性溃疡。目前基于静脉注射五价锑剂的治疗方法，患者依从性低，且可能导致严重不良反应，因此需要寻找新的治疗选择。因此，本研究旨在评估局部应用“智能敷料”作为CL的替代治疗方法。将BALB/c小鼠感染 前鞭毛体。之后，用含有临床使用药物的疏水敷料治疗病变。在病变发展后，进行了以下分析：病变直径测量、血清生化分析、无鞭毛体形式恢复情况评估和组织学分析。未观察到血清参数有显著临床变化。用葡糖胺锑钠浸渍的敷料治疗的组从组织中回收的无鞭毛体数量最少（寄生虫负荷）。用葡糖胺锑钠（腹腔注射）进行的传统治疗也能够降低寄生虫负荷。在测量病变6周后，用喷他脒浸渍的敷料治疗的小鼠病变值最小。病变的代表性组织学特征显示，当分别用葡糖胺锑钠和喷他脒浸渍的敷料治疗小鼠时，巨噬细胞内无或仅有少量无鞭毛体。此处呈现的研究结果表明，局部治疗可能构成CL的一种替代治疗选择。
