Suppr超能文献

枸杞中参与类胡萝卜素生物合成的、和基因的过表达增强了烟草中的类胡萝卜素积累及耐盐性。

Over-Expression of , , and , Genes From Wolfberry for Carotenoid Biosynthesis, Enhanced Carotenoid Accumulation, and Salt Tolerance in Tobacco.

作者信息

Li Chen, Ji Jing, Wang Gang, Li Zhaodi, Wang Yurong, Fan Yajun

机构信息

School of Environmental Science and Engineering, Tianjin University, Tianjin, China.

Division of Biological Sciences, University of California, San Diego, San Diego, CA, United States.

出版信息

Front Plant Sci. 2020 Feb 26;11:119. doi: 10.3389/fpls.2020.00119. eCollection 2020.

Abstract

It is of great importance to combine stress tolerance and plant quality for breeding research. In this study, the role of phytoene desaturase (PDS), ζ-carotene desaturase (ZDS) and carotene isomerase (CRTISO) in the carotenoid biosynthesis are correlated and compared. The three genes were derived from and involved in the desaturation of tetraterpene. Their over-expression significantly increased carotenoid accumulation and enhanced photosynthesis and salt tolerance in transgenic tobacco. Up-regulation of almost all the genes involved in the carotenoid biosynthesis pathway and only significant down-regulation of lycopene ε-cyclase (ε-LCY) gene were detected in those transgenic plants. Under salt stress, proline content, and activities of catalase (CAT), peroxidase (POD) and superoxide dismutase (SOD) were significantly increased, whereas malonaldehyde (MDA) and hydrogen peroxide (HO) accumulated less in the transgenic plants. The genes encoding ascorbate peroxidase (APX), CAT, POD, SOD, and pyrroline-5-carboxylate reductase (P5CR) were shown to responsive up-regulated significantly under the salt stress in the transgenic plants. This study indicated that , , and have the potential to improve carotenoid content and salt tolerance in higher plant breeding.

摘要

将耐逆性与植物品质相结合用于育种研究具有重要意义。在本研究中,对八氢番茄红素去饱和酶(PDS)、ζ-胡萝卜素去饱和酶(ZDS)和胡萝卜素异构酶(CRTISO)在类胡萝卜素生物合成中的作用进行了关联和比较。这三个基因源自并参与四萜的去饱和作用。它们的过表达显著增加了类胡萝卜素积累,并增强了转基因烟草的光合作用和耐盐性。在那些转基因植物中检测到几乎所有参与类胡萝卜素生物合成途径的基因上调,而仅番茄红素ε-环化酶(ε-LCY)基因显著下调。在盐胁迫下,转基因植物中脯氨酸含量以及过氧化氢酶(CAT)、过氧化物酶(POD)和超氧化物歧化酶(SOD)的活性显著增加,而丙二醛(MDA)和过氧化氢(HO)积累较少。编码抗坏血酸过氧化物酶(APX)、CAT、POD、SOD和吡咯啉-5-羧酸还原酶(P5CR)的基因在转基因植物盐胁迫下显示出显著的响应上调。本研究表明,PDS、ZDS和CRTISO在高等植物育种中具有提高类胡萝卜素含量和耐盐性的潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/cee2/7054348/ddc76c91e150/fpls-11-00119-g001.jpg

文献AI研究员

20分钟写一篇综述,助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型,支持多种主流文档格式。

立即体验