Yan Ting, Liang Lu, Yin Ping, Zhou Yang, Sharoba Ashraf Mahdy, Lu Qun, Dong Xingxing, Liu Kun, Connerton Ian F, Li Jinquan
Key Laboratory of Environment Correlative Dietology, College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough, Leicestershire LE12 5RD, UK.
Microorganisms. 2020 Mar 12;8(3):400. doi: 10.3390/microorganisms8030400.
is a leading cause of foodborne diseases, and in recent years, many isolates have exhibited a high level of antibiotic resistance, which has led to huge pressures on public health. Phages are a promising strategy to control food-borne pathogens. In this study, one of our environmental phage isolates, LPSEYT, was to be able to restrict the growth of zoonotic in vitro over a range of multiplicity of infections. Phage LPSEYT exhibited wide-ranging pH and thermal stability and rapid reproductive activity with a short latent period and a large burst size. Phage LPSEYT demonstrated potential efficiency as a biological control agent against in a variety of food matrices, including milk and lettuce. Morphological observation, comparative genomic, and phylogenetic analysis revealed that LPSEYT does not belong to any of the currently identified genera within the family, and we suggest that LPSEYT represents a new genus, the virus. This study contributes a phage database, develops beneficial phage resources, and sheds light on the potential application value of phages LPSEYT on food safety.
是食源性疾病的主要原因，近年来，许多分离株表现出高度的抗生素耐药性，这给公共卫生带来了巨大压力。噬菌体是控制食源性病原体的一种有前景的策略。在本研究中，我们的一种环境噬菌体分离株LPSEYT能够在一系列感染复数范围内体外抑制人畜共患病原体的生长。噬菌体LPSEYT表现出广泛的pH和热稳定性以及快速的繁殖活性，潜伏期短且爆发量高。噬菌体LPSEYT在包括牛奶和生菜在内的多种食品基质中作为针对该病原体的生物控制剂显示出潜在效率。形态学观察、比较基因组学和系统发育分析表明，LPSEYT不属于该噬菌体科目前已鉴定的任何属，我们建议LPSEYT代表一个新属，即该病毒属。本研究贡献了一个噬菌体数据库，开发了有益的噬菌体资源，并揭示了噬菌体LPSEYT在食品安全方面的潜在应用价值。
