宏基因组下一代测序在鹦鹉热衣原体肺炎诊断中的应用：五例报告。
The application of metagenomic next-generation sequencing in diagnosing Chlamydia psittaci pneumonia: a report of five cases.
机构信息
Graduate College of Fujian Medical University, Minhou, Fuzhou, 350108, China.
Department of Respiratory and Critical Care Medicine, Dongfang Hospital of Xiamen University, Fuzhou General Hospital of Fujian Medical University, The 900th Hospital of the Joint Logistic Support Force, PLA, Gulou, Fuzhou, 350025, China.
出版信息
BMC Pulm Med. 2020 Mar 17;20(1):65. doi: 10.1186/s12890-020-1098-x.
BACKGROUND
Chlamydia psittaci pneumonia is a zoonotic infectious disease caused by Chlamydia psittaci. Diagnostic tools, including culture, serologic test and PCR-based methods, are available but prone to false negative results.
CASE PRESENTATION
This report included five cases of Chlamydia psittaci pneumonia. Symptoms and signs common to all 5 cases included fever, coughing, generalized muscle ache, and most notably, inflammatory infiltration of the lungs upon chest CT and X-ray. Metagenomic next-generation sequencing (mNGS) revealed the presence of Chlamydia psittaci in biopsy lung tissue in 3 cases and bronchoalveolar lavage fluid in the remaining 2 cases. Three patients responded to doxycycline plus moxifloxacin; two patients responded to moxifloxacin alone.
CONCLUSIONS
mNGS could be used to diagnose Chlamydia psittaci pneumonia.
背景
鹦鹉热衣原体肺炎是由鹦鹉热衣原体引起的人畜共患传染病。现已有多种诊断方法，包括培养、血清学检测和基于 PCR 的方法，但这些方法容易出现假阴性结果。
病例介绍
本报告共包括 5 例鹦鹉热衣原体肺炎。5 例患者的症状和体征均较为常见，包括发热、咳嗽、全身肌肉疼痛，最显著的特征是胸部 CT 和 X 射线显示肺部炎症浸润。宏基因组下一代测序（mNGS）在 3 例患者的活检肺组织和另外 2 例患者的支气管肺泡灌洗液中均发现了鹦鹉热衣原体。3 例患者对多西环素联合莫西沙星治疗有反应，2 例患者对莫西沙星单独治疗有反应。
结论
mNGS 可用于诊断鹦鹉热衣原体肺炎。
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