Ndede Isaac, Mining Simeon Kipkoech, Patel Kirtika, Wanjala Fredrick Muyoma, Tenge Constance Nalianya
Department of Immunology, Moi University School of Medicine, Eldoret, Kenya.
Department of Biological Sciences, University of Eldoret, Eldoret, Kenya.
Pan Afr Med J. 2019 Dec 19;34:206. doi: 10.11604/pamj.2019.34.206.20244. eCollection 2019.
Burkitt's lymphoma (BL) is a frequent childhood B cell non-Hodgkin's lymphoma (NHL) in equatorial Africa associated with infections. Chronic Epstein Barr virus (EBV) infections can lead to host immune stimulation that may trigger genetic translocation(s), neoplastic transformation and proliferation of B cells. We determined EBV immunoglobulin G (IgG) in sera from participants and EBER-1 in tumour sections in confirmed BL cases at Moi Teaching and Referral Hospital (MTRH). A cross sectional study of children with clinical and histology diagnosis of NHL from whom BL status were confirmed by immunohistochemistry (IHC) was carried out. Epstein Barr virus IgG in sera was determine using Enzyme-linked immunosorbant assay, IHC for EBER-1 and MYC protein in tumour sections. Demographic and clinical information were obtained from questionnaires and hospital files respectively. Ninety three percent of sera were EBV IgG positive of which 31.7% were confirmed as BL. All jaw BL tumours and 86.7% of BL tumours carried EBER-1 antigen. Odds ratio of EBER-1 positive was 1.39, 95% CI: 0.16-12.19 in BL tumours regardless of age or gender. EBV infection among the study participants may be associated with BL, however, EBER-1 and MYC negative in BL tumours suggest alternative BL pathogenesis or variant.
伯基特淋巴瘤（BL）是赤道非洲地区一种常见的儿童B细胞非霍奇金淋巴瘤（NHL），与感染有关。慢性爱泼斯坦-巴尔病毒（EBV）感染可导致宿主免疫刺激，这可能触发B细胞的基因易位、肿瘤转化和增殖。我们在莫伊教学与转诊医院（MTRH）确诊的BL病例中，检测了参与者血清中的EBV免疫球蛋白G（IgG）以及肿瘤切片中的EBER-1。对临床和组织学诊断为NHL且经免疫组织化学（IHC）确诊为BL状态的儿童进行了一项横断面研究。使用酶联免疫吸附测定法检测血清中的爱泼斯坦-巴尔病毒IgG，对肿瘤切片中的EBER-1和MYC蛋白进行免疫组织化学检测。分别从问卷和医院档案中获取人口统计学和临床信息。93%的血清EBV IgG呈阳性，其中31.7%被确诊为BL。所有颌部BL肿瘤和86.7%的BL肿瘤携带EBER-1抗原。无论年龄或性别，BL肿瘤中EBER-1阳性的优势比为1.39，95%置信区间：0.16 - 12.19。研究参与者中的EBV感染可能与BL有关，然而，BL肿瘤中EBER-1和MYC呈阴性提示存在其他BL发病机制或变异型。
