Yi Ruokun, Tan Fang, Zhou Xianrong, Mu Jianfei, Li Lin, Du Xiping, Yang Zhennai, Zhao Xin
Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University (BTBU), Beijing, China.
Chongqing Collaborative Innovation Center for Functional Food, Chongqing Engineering Research Center of Functional Food, Chongqing Engineering Laboratory for Research and Development of Functional Food, Chongqing University of Education, Chongqing, China.
Front Microbiol. 2020 Sep 3;11:573586. doi: 10.3389/fmicb.2020.573586. eCollection 2020.
Probiotics are functional foods that can effectively regulate lipid reduction and maintain body health. In this study, a strain of CQPC04 (LF-CQPC04) isolated from traditional naturally fermented vegetables (Sichuan pickles) was studied, and its effects on lipid reduction in mice, as well as its mechanism of action, were observed. The results of this experiment show that LF-CQPC04 can reduce the abnormal weight gain and abnormal visceral index of mice caused by a high-fat diet. LF-CQPC04 can decrease TG (triglycerides), TC (total cholesterol), LDL-c (low-density lipoprotein cholesterol), AST (aspartate transaminase), ALT (alanine aminotransferase), and AKP (alkaline phosphatase) levels and increase HDL-c (high-density lipoprotein cholesterol) levels in the serum of high-fat mice. LF-CQPC04 can also decrease the levels of inflammatory cytokines, such as IL-6 (interleukin-6), IL-1β (interleukin-1 beta), TNF-α (tumor necrosis factor alpha), and IFN-γ (interferon gamma), and increase IL-4 and IL-10 levels in the serum of high-fat mice. The results of RT-qPCR (real-time quantitative polymerase chain reaction) and western blot experiments show that LF-CQPC04 can also down-regulate the expression of PPAR-γ (peroxisome proliferator-activated receptor gamma), C/EBP-α (CCAAT/enhances binding protein alpha) mRNA, and protein in the liver tissue of high-fat mice, while up-regulating the expression of Cu/Zn-SOD (copper/zinc superoxide dismutase), Mn-SOD (manganese superoxide dismutase), CAT (catalase), CYP7A1 (cholesterol 7 alpha hydroxylase), PPAR-α (peroxisome proliferator-activated receptor alpha), CPT1 (carnitine palmitoyl transferase 1), LPL (lipoprotein lipase), and ABCA1 (ATP-binding cassette transporter A1). Moreover, LF-CQPC04 shows stronger effects in regulating lipid reduction in mice than L-carnitine and commercial LB ( subsp. ) bacteria. LF-CQPC04 is beneficial for lipid reduction in animals and has good probiotic potential.
益生菌是能够有效调节降脂并维持身体健康的功能性食品。在本研究中，对从传统天然发酵蔬菜（四川泡菜）中分离出的一株CQPC04（LF-CQPC04）进行了研究，观察其对小鼠降脂的作用及其作用机制。本实验结果表明，LF-CQPC04可减轻高脂饮食引起的小鼠体重异常增加和内脏指数异常。LF-CQPC04可降低高脂小鼠血清中的甘油三酯（TG）、总胆固醇（TC）、低密度脂蛋白胆固醇（LDL-c）、天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和碱性磷酸酶（AKP）水平，并提高高密度脂蛋白胆固醇（HDL-c）水平。LF-CQPC04还可降低高脂小鼠血清中白细胞介素-6（IL-6）、白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）和干扰素-γ（IFN-γ）等炎症细胞因子的水平，并提高IL-4和IL-10水平。实时定量聚合酶链反应（RT-qPCR）和蛋白质印迹实验结果表明，LF-CQPC04还可下调高脂小鼠肝脏组织中过氧化物酶体增殖物激活受体γ（PPAR-γ）、CCAAT/增强子结合蛋白α（C/EBP-α）mRNA和蛋白的表达，同时上调铜/锌超氧化物歧化酶（Cu/Zn-SOD）、锰超氧化物歧化酶（Mn-SOD）、过氧化氢酶（CAT）、胆固醇7α羟化酶（CYP7A1）、过氧化物酶体增殖物激活受体α（PPAR-α）、肉碱棕榈酰转移酶1（CPT1）、脂蛋白脂肪酶（LPL）和ATP结合盒转运体A1（ABCA1）的表达。此外，LF-CQPC04在调节小鼠降脂方面比左旋肉碱和商业LB（亚种）细菌表现出更强的作用。LF-CQPC０４对动物降脂有益，具有良好的益生菌潜力。
