Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Key Laboratory of Oilseeds Processing of Ministry of Agriculture, Key Laboratory of Biology and Genetic Improvement of Oil Crops of Ministry of Agriculture, PR China; Hubei Key Laboratory of Lipid Chemistry and Nutrition, PR China.
Enshi Autonomous Prefecture Academy of Agricultural Sciences, Enshi, Hubei 445002, PR China.
Food Chem. 2020 Jul 30;319:126547. doi: 10.1016/j.foodchem.2020.126547. Epub 2020 Mar 2.
In this research, a novel ultrasound-assisted extraction (USAE) method using a one-phase solvent (isopropanol, IPA) combined with liquid chromatography-electrospray ionization quadrupole time-of-flight mass spectrometry (LC-ESI-QToF-MS) was developed for exhaustive profiling of egg yolk lipids, which are considered to be essential nutrients that have important functions for human health. Compared with IPA-vortex extraction method, the optimized IPA-USAE method shows better performance in terms of extraction time, lipid losses, lipid recoveries and the number of lipids identified. With this established simple, high throughput and reliable analytical approach, untargeted comprehensive lipid profiling of four kinds of egg yolks was achieved, thus providing a powerful tool for nutritional research of egg lipids. Considering that the lipids contained in egg yolk are quite abundant in both quantity and species, the proposed IPA-USAE combined with LC-ESI-QToF-MS is also potentially applicable to comprehensive extraction and profiling of lipids in other biological samples.
本研究采用单相溶剂（异丙醇，IPA）结合液相色谱-电喷雾电离四极杆飞行时间质谱（LC-ESI-QToF-MS）建立了一种新型的超声辅助提取（USAE）方法，用于全面分析蛋黄脂质，这些脂质被认为是对人体健康有重要功能的必需营养素。与 IPA 涡旋提取法相比，优化的 IPA-USAE 方法在提取时间、脂质损失、脂质回收率和鉴定的脂质数量方面表现出更好的性能。通过建立这种简单、高通量和可靠的分析方法，实现了四种蛋黄的非靶向性全面脂质分析，为蛋黄脂质的营养研究提供了有力工具。考虑到蛋黄中所含的脂质在数量和种类上都非常丰富，所提出的 IPA-USAE 结合 LC-ESI-QToF-MS 也可能适用于其他生物样品中脂质的全面提取和分析。
