Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama 700-8558, Japan.
Department of Preventive Dentistry, Okayama University Hospital, Okayama 700-8558, Japan.
Int J Environ Res Public Health. 2020 Mar 9;17(5):1764. doi: 10.3390/ijerph17051764.
The purpose of this prospective cohort study was to investigate the influence of the salivary microbiome on the worsening of the periodontal health status among Japanese young adults. We assessed the data of systemically healthy and non-smoking young (18-22 years) university students ( = 457) from Okayama University at baseline (2013) and follow-up (2016). The worsening group was defined based on an increase in the percentage of bleeding on probing (%BOP) or an increase in probing pocket depth (PPD) from <4 mm to ≥4 mm. Unstimulated saliva samples were randomly collected from 69 students for microbiome analysis at follow-up. The salivary microbiome was assessed through 16S rRNA metagenomic sequencing. The type of community in the salivary microbiome clustered by statistical analysis and diversity was not significantly associated with the worsening of the periodontal health status in cases of increasing %BOP and PPD ( > 0.05). The prevalence of some species was significantly higher in the worsening group than in the non-worsening group ( < 0.05) in both cases. The worsening of the periodontal health status was associated with some species, but not the type of community and diversity in the salivary microbiome among Japanese young adults.
本前瞻性队列研究旨在探讨唾液微生物组对日本年轻成年人牙周健康状况恶化的影响。我们评估了冈山大学健康且不吸烟的年轻（18-22 岁）大学生（n=457）的基线（2013 年）和随访（2016 年）数据。根据探诊出血百分比（%BOP）或探诊牙周袋深度（PPD）从<4mm 增加到≥4mm，将恶化组定义为%BOP 或 PPD 增加。在随访时，从 69 名学生中随机采集未刺激唾液样本进行微生物组分析。通过 16S rRNA 宏基因组测序评估唾液微生物组。通过统计分析聚类唾液微生物组的群落类型和多样性与 %BOP 和 PPD 增加时（>0.05）牙周健康状况恶化无显著相关性。在两种情况下，恶化组的某些物种的流行率均显著高于非恶化组（<0.05）。在日本年轻成年人中，牙周健康状况的恶化与唾液微生物组中的某些物种有关，但与群落类型和多样性无关。
