Department of Preventive Dentistry, Okayama University Hospital, Okayama 700-8558, Japan.
Advanced Research Center for Oral and Craniofacial Sciences, Okayama University Dental School, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan.
Int J Environ Res Public Health. 2020 May 25;17(10):3713. doi: 10.3390/ijerph17103713.
The purpose of this 3-year prospective cohort study was to explore the relationship between an increase in dental caries and oral microbiome among Japanese university students. We analyzed 487 students who volunteered to receive oral examinations and answer baseline (2013) and follow-up (2016) questionnaires. Of these students, salivary samples were randomly collected from 55 students at follow-up and analyzed using next-generation sequencing. Students were divided into two groups: increased group (Δdecayed, missing, and filled teeth (ΔDMFT) score increased during the 3-year period) and non-increased group (ΔDMFT did not increase). Thirteen phyla, 21 classes, 32 orders, 48 families, 72 genera, and 156 species were identified. Microbial diversity in the increased group ( = 14) was similar to that in the non-increased group ( = 41). Relative abundances of the family ( = 0.007) and genera ( = 0.007) and ( = 0.039) were enriched in the increased group compared with the non-increased group. Some bacterial taxonomic clades were differentially present between the two groups. These results may contribute to the development of new dental caries prevention strategies, including the development of detection kits and enlightenment activities for these bacteria.
本为期 3 年的前瞻性队列研究旨在探讨日本大学生中龋齿增加与口腔微生物组之间的关系。我们分析了 487 名自愿接受口腔检查并回答基线（2013 年）和随访（2016 年）问卷的学生。其中，55 名学生在随访时随机采集唾液样本，并用下一代测序进行分析。学生分为两组：增加组（ΔDMFT 评分在 3 年内增加）和未增加组（ΔDMFT 未增加）。鉴定出 13 个门、21 个纲、32 个目、48 个科、72 个属和 156 个种。增加组（ = 14）和未增加组（ = 41）的微生物多样性相似。与未增加组相比，增加组中家族 （ = 0.007）和属 （ = 0.007）和 （ = 0.039）的相对丰度增加。两组之间存在一些细菌分类群的差异。这些结果可能有助于开发新的龋齿预防策略，包括开发这些细菌的检测试剂盒和启发活动。
