Ma Fanshu, Zhang Erqin, Li Qingmei, Xu Qianru, Ou Jiquan, Yin Heng, Li Kunpeng, Wang Li, Zhao Xiangyue, Niu Xiangxiang, Li Xueyang, Zhang Shenli, Wang Yanan, Deng Ruiguang, Zhou Enmin, Zhang Gaiping
Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Northwest A&F University, Yangling 712100, China.
Department of Preventive Veterinary Medicine, College of Animal Science and Veterinary Medicine, Henan Agricultural University, Zhengzhou 450002, China.
Vaccines (Basel). 2020 Mar 9;8(1):122. doi: 10.3390/vaccines8010122.
Newcastle disease (ND) is a highly contagious avian disease, causing considerable economic losses to the poultry industry. To obtain a safe, inexpensive, and effective ND vaccine to meet the international trade requirements of differentiating infected from vaccinated animals (DIVA), here we report the production of Oryza sativa recombinant fusion (F) protein in stably transformed transgenic rice seeds via agroinfiltration. The F protein expression level was enhanced 3.6-fold with a genetic background in low glutelin. Inoculation of plant-produced F antigen into Specific Pathogen Free (SPF) chickens markedly elicited neutralizing antibody responses against homologous and heterologous ND virus strains. Two doses of 4.5 μg fully protected chickens from a lethal ND challenge without any clinical symptoms. The mean weight gain of F protein-immunized chickens within 15 days after challenge was significantly higher than that of traditional whole virus vaccine-immunized chickens, thereby obtaining higher economic benefits. Moreover, the sera from the chickens vaccinated with the plant-produced F vaccine did not show reactivity in an immunochromatographic strip targeting the haemagglutinin-neuraminidase protein (HN) protein, and DIVA could be achieved within 10 minutes. Our results demonstrate that the plant-derived F vaccine along with immunochromatographic strips could be useful in the implementation of an NDV eradication program.
新城疫（ND）是一种高度传染性的禽类疾病，给家禽业造成了巨大的经济损失。为了获得一种安全、廉价且有效的新城疫疫苗，以满足区分感染动物和免疫动物（DIVA）的国际贸易要求，在此我们报告通过农杆菌浸润在稳定转化的转基因水稻种子中生产水稻重组融合（F）蛋白。在低谷蛋白的遗传背景下，F蛋白表达水平提高了3.6倍。将植物产生的F抗原接种到无特定病原体（SPF）鸡中，显著引发了针对同源和异源新城疫病毒株的中和抗体反应。两剂4.5μg的F蛋白可完全保护鸡免受致死性新城疫攻击，且无任何临床症状。攻毒后15天内，F蛋白免疫鸡的平均体重增加显著高于传统全病毒疫苗免疫鸡，从而获得更高的经济效益。此外，用植物产生的F疫苗免疫的鸡的血清在针对血凝素 - 神经氨酸酶蛋白（HN）的免疫层析条上不显示反应性，并且可在10分钟内实现DIVA。我们的结果表明，植物源F疫苗与免疫层析条可用于实施新城疫病毒根除计划。
