Department of Integrative Biology, University of Texas at Austin, Austin, TX 78712, USA.
Centre of Excellence in Bionanoscience Research, Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia.
Int J Mol Sci. 2020 Mar 13;21(6):1959. doi: 10.3390/ijms21061959.
The genus is the largest of the tribe Trifolieae in the subfamily Papilionoideae (Fabaceae). The paucity of mitochondrial genome (mitogenome) sequences has hindered comparative analyses among the three genomic compartments of the plant cell (nucleus, mitochondrion and plastid). We assembled four mitogenomes from the two subgenera ( and ) of the genus. The four mitogenomes were compact (294,911-348,724 bp in length) and contained limited repetitive (6.6-8.6%) DNA. Comparison of organelle repeat content highlighted the distinct evolutionary trajectory of plastid genomes in a subset of species. Intracellular gene transfer (IGT) was analyzed among the three genomic compartments revealing functional transfer of mitochondrial to nuclear genome along with other IGT events. Phylogenetic analysis based on mitochondrial and nuclear sequences revealed that the functional transfer in Trifolieae was independent from the event that occurred in robinioid clade that includes genus . A novel, independent fission event of in was identified, caused by a 59 bp deletion. Fissions of this gene reported previously in land plants were reassessed and compared with .
是豆科蝶形花亚科车轴草族中最大的属。由于植物细胞的三个基因组区（核、线粒体和质体）的线粒体基因组（mitogenome）序列很少，因此妨碍了它们之间的比较分析。我们从该属的两个亚属（和）中组装了四个线粒体基因组。这四个 线粒体基因组紧凑（长 294911-348724bp），含有有限的重复（6.6-8.6%）DNA。细胞器重复含量的比较突出了一组 物种中质体基因组的独特进化轨迹。对三个基因组区之间的细胞内基因转移（IGT）进行了分析，结果表明线粒体 向核基因组的功能转移以及其他 IGT 事件。基于线粒体和核 序列的系统发育分析表明，车轴草族中的功能转移与包括属 在内的 robinioid 分支发生的事件无关。在 中发现了一个新的、独立的 分裂事件，由 59bp 的缺失引起。以前在陆生植物中报道的这个基因的分裂事件被重新评估，并与 进行了比较。
