Balaji Siripireddy, Mandal Badal Kumar, Vinod Kumar Reddy L, Sen Dwaipayan
Trace Elements Speciation Research Laboratory, Department of Chemistry, School of Advanced Sciences, Vellore Institute of Technology, Vellore 632014, India.
Cellular and Molecular Therapeutics Laboratory, Centre for Biomaterials, Cellular and Molecular Theranostics, Vellore Institute of Technology, Vellore 632014, India.
Bioengineering (Basel). 2020 Mar 13;7(1):26. doi: 10.3390/bioengineering7010026.
Ceria nanoparticles (CeO NPs) are generally considered in various functional applications, such as catalysts in fuel cells, sensors, and antioxidant and oxidase-like enzymes in the biological environment. The CeO NPs were synthesized using the leaf extract-mediated hydrothermal technique. The synthesized NPs were characterized by various analytical instruments including powder X-ray diffractometer (PXRD), scanning electron microscope (SEM), transmission electron microscope (TEM) and dynamic light scattering (DLS) analysis. The XRD results showed an average NPs sizes of 13.7 nm. Cytotoxic study results showed an IC value of 45.5 µg/L for A549 and 58.2 µg/L for HCT 116, indicating that CeO NPs are more toxic to A549 compared to HCT116 cell lines. The generation of ROS was responsible for its cytotoxic activity against cancer cell lines. Specific surface area (40.96 m/g) and pore diameter (7.8 nm) were measured using Brunauer-Emmett-Teller (BET) nitrogen adsorption-desorption isotherms. CeO NPs with a high surface area were used as photocatalyst in degrading sunset yellow (SY) dye under UV-irradiation and 97.3% of the dye was degraded within 90 min. These results suggest that the synthesized CeO NPs could be used as a good photocatalyst as well as a cytotoxic agent against human cancer cell lines.
二氧化铈纳米颗粒（CeO NPs）通常被用于各种功能应用中，例如燃料电池中的催化剂、传感器以及生物环境中的抗氧化剂和类氧化酶。采用叶提取物介导的水热技术合成了CeO NPs。使用包括粉末X射线衍射仪（PXRD）、扫描电子显微镜（SEM）、透射电子显微镜（TEM）和动态光散射（DLS）分析在内的各种分析仪器对合成的纳米颗粒进行了表征。XRD结果显示纳米颗粒的平均尺寸为13.7纳米。细胞毒性研究结果表明，A549细胞系的IC值为45.5微克/升，HCT 116细胞系的IC值为58.2微克/升，这表明与HCT116细胞系相比，CeO NPs对A549细胞的毒性更大。活性氧的产生是其对癌细胞系具有细胞毒性活性的原因。使用布鲁诺尔-埃米特-泰勒（BET）氮吸附-解吸等温线测量了比表面积（40.96平方米/克）和孔径（7.8纳米）。具有高表面积的CeO NPs在紫外光照射下用作降解日落黄（SY）染料的光催化剂，90分钟内97.3%的染料被降解。这些结果表明，合成的CeO NPs既可以用作良好的光催化剂，也可以用作针对人类癌细胞系的细胞毒性剂。
