免疫印迹法在血清蛋白表型分析中的应用——参考血迹的α2HS-糖蛋白（AHS）分型

Application of immunoblotting to serum protein phenotyping with reference to alpha 2HS-glycoprotein (AHS) typing of bloodstains.

作者信息

Tamaki Y, Fukuda M, Nishimukai H, Kishida T

出版信息

Z Rechtsmed. 1985;95(3):153-8. doi: 10.1007/BF00201072.

DOI:10.1007/BF00201072

Abstract

Sera and bloodstain extracts were subjected to isoelectric focusing in polyacrylamide gels. The focused proteins were transferred to nitrocellulose membranes by diffusion or electrophoretically, then allowed to react with specific antiserum and, after washing, with peroxidase-labeled anti-rabbit IgG. The immune complexes formed on the membranes were detected with 4-chloro-1-naphthol and hydrogen peroxide. Serum group-specific component, alpha 2HS-glycoprotein, the sixth and the seventh component of complement, factor 13B, and plasminogen could be phenotyped with high sensitivity. Bloodstains as old as 6 months could be correctly typed for alpha 2HS-glycoprotein by the blotting technique.

摘要

血清和血迹提取物在聚丙烯酰胺凝胶中进行等电聚焦。聚焦后的蛋白质通过扩散或电泳转移至硝酸纤维素膜上，然后与特异性抗血清反应，洗涤后再与过氧化物酶标记的抗兔IgG反应。用4-氯-1-萘酚和过氧化氢检测膜上形成的免疫复合物。血清组特异性成分、α2HS-糖蛋白、补体第六和第七成分、因子13B和纤溶酶原可被高灵敏度地表型分析。通过印迹技术，6个月之久的血迹的α2HS-糖蛋白也能被正确分型。