Chávez-Morales Yadid, Jiménez-Ferrer Enrique, Martínez-Hernández Gabriela Belen, Tortoriello Jaime, Román-Ramos Rubén, Zamilpa Alejandro, Herrera-Ruiz Maribel
Centro de Investigación Biomédica del Sur, Instituto Mexicano del Seguro Social (IMSS), Argentina 1, 62790 Xochitepec, Morelos, México.
Doctorado en Ciencias Biológicas y de la Salud, División de Ciencias Biológicas y de la Salud, Universidad.
Iran J Pharm Res. 2019 Fall;18(4):1931-1946. doi: 10.22037/ijpr.2019.1100883.
Depression affects more than 300 million people worldwide, represents one of the leading causes of disability worldwide. Depression treatment is based on the use of tricyclic antidepressants, selective serotonin reuptake inhibitors. These drugs, although clinically effective, have also been shown to have delayed onset activity and produce significant adverse side effects. Medicinal plants are presented as a source of study in the search for therapies. This study was aimed to assess the antidepressant effect (on forced swimming test -FST- and tail suspension test -TST-) of different fractions and tiliroside from . The organic fractions (FAC1-1, FAC1-2) and aqueous fractions (FAqC2-1, FAqC2-3) were obtained by column chromatography and the HPLC analysis allowed the standardization based on the concentration (mg/g) of several compounds: FAqC2-1 with tiliroside 20, quercitrin 41.7, and quercetin glucoside 73.8; FAqC2-3 with tiliroside 2.4, quercitrin 16.6 and 7-O-luteolin glucoside 35.9; FAC1-1 caffeic acid was quantified with 7.87 ; FAC1-2 with tiliroside 24.7 and quercitrin 19.8. Each fraction was tested in ICR mice at different dose in the FST and TST, as well as in the open field test (OFT); tiliroside was isolated and tested in such assays (at 0.05, 0.1, 0.5, and 1.0 mg/kg). All fractions were active, the better was FAC1-2, and induced a dose-dependent effect on FST with an ED= 2.59 mg/kg and Emax = 175.4 sec; with a sedative effect in OFT. Tiliroside with like-antidepressant activity, showed a dose-response behavior (ED= 0.04 mg/kg and Emax = 121.42 sec for FST; ED= 0.014 mg/kg and Emax = 78.28 sec for TST).
抑郁症影响着全球超过3亿人，是全球致残的主要原因之一。抑郁症的治疗基于使用三环类抗抑郁药、选择性5-羟色胺再摄取抑制剂。这些药物虽然在临床上有效，但也被证明起效延迟且会产生显著的不良副作用。药用植物作为寻找治疗方法的研究来源而被提出。本研究旨在评估不同提取物及椴树苷（从……中提取）对强迫游泳试验（FST）和悬尾试验（TST）的抗抑郁作用。通过柱色谱法获得有机提取物（FAC1 - 1、FAC1 - 2）和水提取物（FAqC2 - 1、FAqC2 - 3），高效液相色谱分析基于几种化合物的浓度（mg/g）实现标准化：FAqC2 - 1含有椴树苷20、槲皮苷41.7和槲皮素葡萄糖苷73.8；FAqC2 - 3含有椴树苷2.4、槲皮苷16.6和7 - O - 木犀草素葡萄糖苷35.9；FAC1 - 1中咖啡酸的含量为7.87 ；FAC1 - 2含有椴树苷24.7和槲皮苷19.8。每个提取物在FST、TST以及旷场试验（OFT）中以不同剂量对ICR小鼠进行测试；分离出椴树苷并在这些试验中进行测试（剂量为0.05、0.1、0.5和1.0 mg/kg）。所有提取物均有活性，其中效果最佳的是FAC1 - 2，对FST产生剂量依赖性效应，ED = 2.59 mg/kg，Emax = 175.4秒；在OFT中有镇静作用。具有类似抗抑郁活性的椴树苷表现出剂量反应行为（FST的ED = 0.04 mg/kg，Emax = 121.42秒；TST的ED = 0.014 mg/kg，Emax = 78.28秒）。
