Shi Pengfei, Cao Zhigang, Cheng Yuening, Cheng Shipeng, Yi Li
Institute of Special Economic Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun, China.
Front Vet Sci. 2020 Feb 28;7:47. doi: 10.3389/fvets.2020.00047. eCollection 2020.
Canine distemper virus (CDV) belongs to the genus of the Paramyxoviridae family, which causes a threat to the domestic dog and fur-animal industry. Hemagglutinin protein is a major membrane protein of the vital molecular factor in CDV tropism, also known to induce hosts to produce neutralizing antibodies. In the current study, we prepared two monoclonal antibodies, 1A5 and 2B8, against the H protein of the CDV-PS strain. A series of partially overlapping synthetic peptides covering the hemagglutinin protein (amino acids 50-204) were screened to define the linear epitope identified by 1A5 and 2B8 mAbs. QKTNFFNPNREFDFR (F8) and ARGDIFPPY (F14-1) are minimal linear epitopes recognized by 1A5 and 2B8 mAbs, respectively. Further investigations revealed that F8 is conserved in different CDV strains; however, F14-1 contains mutant residues 178, 179, and 180. The epitopes F8 and F14-1 localized at the surface of hemagglutinin protein in a three-dimensional (3D) structure. CDV-infected dog serum can also recognize the identified B-cell epitopes.
犬瘟热病毒（CDV）属于副粘病毒科，对家犬和毛皮动物产业构成威胁。血凝素蛋白是CDV嗜性的关键分子因子中的主要膜蛋白，也已知可诱导宿主产生中和抗体。在本研究中，我们制备了两种针对CDV-PS株H蛋白的单克隆抗体1A5和2B8。筛选了一系列覆盖血凝素蛋白（氨基酸50-204）的部分重叠合成肽，以确定由1A5和2B8单克隆抗体识别的线性表位。QKTNFFNPNREFDFR（F8）和ARGDIFPPY（F14-1）分别是1A5和2B8单克隆抗体识别的最小线性表位。进一步研究表明，F8在不同的CDV株中保守；然而，F14-1含有178、179和180位的突变残基。表位F8和F14-1在三维（3D）结构中位于血凝素蛋白表面。感染CDV的犬血清也能识别鉴定出的B细胞表位。
