Department of Infection Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, UK.
Cell Microbiol. 2020 Apr;22(4):e13173. doi: 10.1111/cmi.13173.
Investigation of cytoskeleton during bacterial infection has significantly contributed to both cell and infection biology. Bacterial pathogens Listeria monocytogenes and Shigella flexneri are widely recognised as paradigms for investigation of the cytoskeleton during bacterial entry, actin-based motility, and cell-autonomous immunity. At the turn of the century, septins were a poorly understood component of the cytoskeleton mostly studied in the context of yeast cell division and human cancer. In 2002, a screen performed in the laboratory of Pascale Cossart identified septin family member MSF (MLL septin-like fusion, now called SEPT9) associated with L. monocytogenes entry into human epithelial cells. These findings inspired the investigation of septins during L. monocytogenes and S. flexneri infection at the Institut Pasteur, illuminating important roles for septins in host-microbe interactions. In this review, we revisit the history of septin biology and bacterial infection, and discuss how the comparative study of L. monocytogenes and S. flexneri has been instrumental to understand septin roles in cellular homeostasis and host defence.
在细菌感染过程中对细胞骨架的研究极大地促进了细胞和感染生物学的发展。细菌病原体李斯特菌（Listeria monocytogenes）和福氏志贺菌（Shigella flexneri）被广泛认为是研究细菌入侵、肌动蛋白基础运动和细胞自主免疫过程中细胞骨架的典范。在本世纪初， septins 是细胞骨架中一个了解甚少的组成部分，主要在酵母细胞分裂和人类癌症的背景下进行研究。2002 年，Pascale Cossart 实验室进行的一项筛选发现了 septin 家族成员 MSF（MLL septin-like fusion，现在称为 SEPT9）与李斯特菌进入人上皮细胞有关。这些发现激发了人们在巴斯德研究所对李斯特菌和福氏志贺菌感染过程中 septins 的研究，阐明了 septins 在宿主-微生物相互作用中的重要作用。在这篇综述中，我们回顾了 septin 生物学和细菌感染的历史，并讨论了比较研究李斯特菌和福氏志贺菌如何有助于理解 septins 在细胞内稳态和宿主防御中的作用。
