两种瑞香科植物的DNA条形码研究：Royle和（L.）Endl.
DNA Barcoding of Two Thymelaeaceae Species: Royle and (L.) Endl.
作者信息
Alkaraki Almuthanna K, Aldmoor Maisam A, Lahham Jamil N, Awad Mohammed
机构信息
Department of Biological Sciences, Faculty of Science, Yarmouk University, Irbid 21163, Jordan.
Department of Biotechnology, Faculty of Agriculture, Al-Azhar University, Cairo 11651, Egypt.
出版信息
Plants (Basel). 2021 Oct 16;10(10):2199. doi: 10.3390/plants10102199.
Royle and (L.) Endl both belong to the family. Both species are used traditionally to treat several diseases along with various daily applications by Jordanian Bedouins. Traditionally, those species are identified through personal proficiency, which could be misleading due to human errors or lack of expertise. This study aims to investigate an effective DNA barcoding method to identify and characterize Royle and plant species at the molecular level. Royle and were collected from the ancient city of Petra in the Southern part of Jordan. Sequences of candidate DNA barcodes were amplified (, , and ), sequenced, and aligned to the blastn database. Moreover, the obtained sequences were compared with available sequences of related species at the GenBank database. Our results showed that DNA barcoding successfully identifies the two plant species using any of chloroplast genes (, , or ). The results emphasize the ability of DNA barcoding for identifying and characterizing different plant species through the recruitment of different barcode loci in molecular identification.
罗伊尔氏（物种名）和（L.）恩德尔氏（物种名）都属于（某个科名未给出）科。这两个物种在传统上被约旦贝都因人用于治疗多种疾病以及各种日常用途。传统上，这些物种是通过个人经验来识别的，由于人为错误或缺乏专业知识，这可能会产生误导。本研究旨在研究一种有效的DNA条形码方法，以在分子水平上识别和表征罗伊尔氏和（物种名未给出）植物物种。罗伊尔氏和（物种名未给出）是从约旦南部的古城佩特拉采集的。候选DNA条形码序列被扩增（（基因名未给出）、（基因名未给出）和（基因名未给出））、测序，并与blastn数据库进行比对。此外，将获得的序列与GenBank数据库中相关物种的可用序列进行比较。我们的结果表明，DNA条形码使用任何一种叶绿体基因（（基因名未给出）、（基因名未给出）或（基因名未给出））都能成功识别这两种植物物种。结果强调了DNA条形码通过在分子鉴定中使用不同的条形码位点来识别和表征不同植物物种的能力。
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