Siu Wing Sum, Shiu Hoi Ting, Shum Wai Ting, Ko Chun Hay, Lau Clara Bik San, Hung Leung Kim, Leung Ping Chung
Institute of Chinese Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong China.
State Key Laboratory of Research on Bioactivities and Clinical Applications of Medicinal Plants, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong China.
J Tradit Chin Med. 2019 Dec;39(6):853-860.
To investigate the efficacy on the combination of oral strontium ranelate (SrR) with a topical Chinese herbal paste on facilitation of fracture healing.
An open fracture was created at the mid-shaft of the right tibia of rat. A herbal paste called CDR containing Honghua (Flos Carthami), Chuanxuduan (Radix Dipsaci Asperoidis) and Dahuang (Radix Et Rhizoma Rhei Palmati) was prepared. The rats were treated with either CDR topically on the fracture site, or SrR orally, or their combinations. Bone turnover biochemical markers in serum were measured. Microarchitecture of the fracture was analyzed using micro-CT after 14 and 28 d, followed by histomorphometrical analysis.
Micro-computed tomography analysis revealed that the combined treatment of CDR with 600 mg/g SrR significantly increased the total callus density, mineralized callus volume fraction, mineralized callus mineral content and mineralized callus density of the callus after 28 d of treatment. This result was consistent with the histomorphometrical analysis on the osteoid volume. Analysis of biochemical markers showed that the combined treatments reduced the bone resorption that occurs temporarily after fracture.
This study demonstrated that the combined treatment of oral SrR and topical CDR is effective to promote fracture healing by their additive effect on promoting bone formation and retarding bone resorption.
探讨口服雷奈酸锶（SrR）与外用中药膏剂联合应用促进骨折愈合的疗效。
在大鼠右胫骨干中段制造开放性骨折。制备一种名为CDR的中药膏剂，其含有红花、川续断和大黄。对大鼠分别进行如下处理：在骨折部位外用CDR、口服SrR或二者联合应用。检测血清中骨转换生化标志物。在第14天和第28天后，使用微型计算机断层扫描（micro-CT）分析骨折的微观结构，随后进行组织形态计量学分析。
微型计算机断层扫描分析显示，CDR与600 mg/g SrR联合治疗在治疗28天后显著增加了骨痂的总密度、矿化骨痂体积分数、矿化骨痂矿物质含量和矿化骨痂密度。该结果与对类骨质体积的组织形态计量学分析一致。生化标志物分析表明，联合治疗减少了骨折后暂时出现的骨吸收。
本研究表明，口服SrR与外用CDR联合治疗通过促进骨形成和抑制骨吸收的相加作用有效促进骨折愈合。
