Microbiology, Virology and Biotechnology Section, Department of Genetics, Microbiology and Statistics, Faculty of Biology, University of Barcelona, Av. Diagonal 643, 08028 Barcelona, Spain.
Viruses. 2020 Mar 16;12(3):318. doi: 10.3390/v12030318.
Rotavirus A (RVA) is the most common virus associated with infantile gastroenteritisworldwide, being a public health threat, as it is excreted in large amounts in stool and can persist inthe environment for extended periods. In this study, we performed the detection of RVA and humanadenovirus (HAdV) by TaqMan qPCR and assessed the circulation of RVA genotypes in threewastewater treatment plants (WWTPs) between 2015 and 2016 in Catalonia, Spain. RVA wasdetected in 90% and HAdV in 100% of the WWTP samples, with viral loads ranging between 3.96 ×104 and 3.30 × 108 RT-PCR Units/L and 9.51 × 104 and 1.16 × 106 genomic copies/L, respectively. RVAVP7 and VP4 gene analysis revealed the circulation of G2, G3, G9, G12, P[4], P[8], P[9] and P[10].Nucleotide sequencing (VP6 fragment) showed the circulation of I1 and I2 genotypes, commonlyassociated with human, bovine and porcine strains. It is important to mention that the RVA strainsisolated from the WWTPs were different from those recovered from piglets and calves living in thesame area of single sampling in 2016. These data highlight the importance of monitoring watermatrices for RVA epidemiology and may be a useful tool to evaluate and predict possibleemergence/reemergence of uncommon strains in a region.
轮状病毒 A（RVA）是与全世界婴幼儿肠胃炎相关的最常见病毒，是一种公共卫生威胁，因为它在粪便中大量排泄，并能在环境中持续存在很长时间。在本研究中，我们通过 TaqMan qPCR 检测了轮状病毒和人腺病毒（HAdV），并评估了 2015 年至 2016 年期间西班牙加泰罗尼亚三个污水处理厂（WWTP）中 RVA 基因型的循环情况。在 90%的 WWTP 样本中检测到 RVA，在 100%的 WWTP 样本中检测到 HAdV，病毒载量在 3.96×104 和 3.30×108 RT-PCR 单位/L 之间，9.51×104 和 1.16×106 基因组拷贝/L 之间。RVA VP7 和 VP4 基因分析显示 G2、G3、G9、G12、P[4]、P[8]、P[9]和 P[10]的循环。核苷酸测序（VP6 片段）显示 I1 和 I2 基因型的循环，通常与人类、牛和猪株有关。值得一提的是，从 WWTP 分离的 RVA 株与 2016 年在同一采样区从仔猪和犊牛中分离的株不同。这些数据突出表明监测水基质中 RVA 流行病学的重要性，并且可能是评估和预测一个地区罕见菌株可能出现/再次出现的有用工具。
