Departament de Bioquímica i Biotecnologia, MoBioFood Research Group, Universitat Rovira I Virgili, 43007, Tarragona, Spain.
Servei de Cirurgia General i de l'Aparell Digestiu, Hospital Universitari Joan XXIII, Tarragona, Spain.
Eur J Nutr. 2021 Feb;60(1):79-88. doi: 10.1007/s00394-020-02222-3. Epub 2020 Mar 18.
Anti-inflammatory and barrier-protective properties have been attributed to proanthocyanidins in the context of intestinal dysfunction, however little information is available about the impact of these phytochemicals on intestinal barrier integrity and immune response in the human. Here we assessed the putative protective properties of a grape-seed proanthocyanidin extract (GSPE) against dextran sodium sulfate (DSS)-induced acute dysfunction of the human colon in an Ussing chamber system.
Human proximal and distal colon tissues from colectomized patients were submitted ex vivo for a 30-min preventive GSPE treatment (50 or 200 µg mL) followed by 1-h incubation with DSS (12% w v). Transepithelial electrical resistance (TEER), permeation of a fluorescently-labeled dextran (FD4) and proinflammatory cytokine release [tumor necrosis factor (TNF)-α and interleukin (IL)-1β] of colonic tissues were determined.
DSS reduced TEER (45-52%) in both the proximal and distal colon; however, significant increments in FD4 permeation (fourfold) and TNF-α release (61%) were observed only in the proximal colon. The preventive GSPE treatment decreased DSS-induced TEER loss (20-32%), FD4 permeation (66-73%) and TNF-α release (22-33%) of the proximal colon dose-dependently. The distal colon was not responsive to the preventive treatment but showed a reduction in IL-1β release below basal levels with the highest GSPE concentration.
Our results demonstrate potential preventive effects of GSPE on human colon dysfunction. Further studies are required to test whether administering GSPE could be a complementary therapeutic approach in colonic dysfunction associated with metabolic disorders and inflammatory bowel disease.
在肠道功能障碍的情况下，原花青素具有抗炎和保护屏障的特性，但关于这些植物化学物质对人类肠道屏障完整性和免疫反应的影响，信息很少。在这里，我们评估了葡萄籽原花青素提取物（GSPE）对葡聚糖硫酸钠（DSS）诱导的人类结肠急性功能障碍的潜在保护特性，使用 Ussing 室系统进行实验。
从结肠切除术患者中采集近端和远端结肠组织，进行离体实验，先进行 30 分钟的预防性 GSPE 处理（50 或 200μg/mL），然后用 DSS（12%w/v）孵育 1 小时。测定跨上皮电阻（TEER）、荧光标记的葡聚糖（FD4）的渗透和结肠组织中促炎细胞因子的释放（肿瘤坏死因子（TNF）-α和白细胞介素（IL）-1β）。
DSS 降低了近端和远端结肠的 TEER（45-52%）；然而，仅在近端结肠观察到 FD4 渗透（增加四倍）和 TNF-α释放（增加 61%）显著增加。预防性 GSPE 处理剂量依赖性地降低了 DSS 诱导的近端结肠 TEER 丧失（20-32%）、FD4 渗透（66-73%）和 TNF-α释放（22-33%）。远端结肠对预防性治疗没有反应，但在最高 GSPE 浓度下，IL-1β的释放低于基础水平。
我们的结果表明 GSPE 对人类结肠功能障碍具有潜在的预防作用。需要进一步的研究来测试是否给予 GSPE 可以成为与代谢紊乱和炎症性肠病相关的结肠功能障碍的一种补充治疗方法。
