Zhang Hao, Zhao Xiaobo, Sun Quanxi, Yan Caixia, Wang Juan, Yuan Cuiling, Li Chunjuan, Shan Shihua, Liu Fengzhen
State Key Laboratory of Crop Biology and College of Agronomy, Shandong Agricultural University, Tai'an, Shandong 271018, China.
Shandong Peanut Research Institute, Qingdao, Shandong 266000, China.
Int J Genomics. 2020 Feb 26;2020:6524093. doi: 10.1155/2020/6524093. eCollection 2020.
Abiotic stresses comprise all nonliving factors, such as soil salinity, drought, extreme temperatures, and metal toxicity, posing a serious threat to agriculture and affecting the plant production around the world. Peanut ( L.) is one of the most important crops for vegetable oil, proteins, minerals, and vitamins in the world. Therefore, it is of importance to understand the molecular mechanism of peanut against salt stress. Six transcriptome sequencing libraries including 24-hour salt treatments and control samples were constructed from the young leaves of peanut. A comprehensive analysis between two groups detected 3,425 differentially expressed genes (DEGs) including 2,013 upregulated genes and 1,412 downregulated genes. Of these DEGs, 141 transcription factors (TFs) mainly consisting of MYB, AP2/ERF, WRKY, bHLH, and HSF were identified in response to salinity stress. Further, GO categories of the DEGs highly related to regulation of cell growth, cell periphery, sustained external encapsulating structure, cell wall organization or biogenesis, antioxidant activity, and peroxidase activity were significantly enriched for upregulated DEGs. The function of downregulated DEGs was mainly enriched in regulation of metabolic processes, oxidoreductase activity, and catalytic activity. Fourteen DEGs with response to salt tolerance were validated by real-time PCR. Taken together, the identification of DEGs' response to salt tolerance of cultivated peanut will provide a solid foundation for improving salt-tolerant peanut genetic manipulation in the future.
非生物胁迫包括所有非生物因素，如土壤盐分、干旱、极端温度和金属毒性，对农业构成严重威胁并影响全球的植物生产。花生是世界上植物油、蛋白质、矿物质和维生素最重要的作物之一。因此，了解花生抗盐胁迫的分子机制具有重要意义。从花生幼叶中构建了包括24小时盐处理和对照样本的6个转录组测序文库。两组之间的综合分析检测到3425个差异表达基因（DEG），其中包括2013个上调基因和1412个下调基因。在这些DEG中，鉴定出141个转录因子（TF），主要由MYB、AP2/ERF、WRKY、bHLH和HSF组成，以响应盐胁迫。此外，上调的DEG中与细胞生长调节、细胞周边、持续外部包封结构、细胞壁组织或生物合成、抗氧化活性和过氧化物酶活性高度相关的GO类别显著富集。下调的DEG的功能主要富集在代谢过程调节、氧化还原酶活性和催化活性方面。通过实时PCR验证了14个对耐盐性有响应的DEG。综上所述，鉴定栽培花生DEG对耐盐性的响应将为未来改进耐盐花生基因操作提供坚实基础。
