Tang Haohong, Yuan Cuiling, Shi Haonan, Liu Feng, Shan Shihua, Wang Zhijun, Sun Quanxi, Sun Jie
Key Laboratory of Oasis Eco-Agriculture, College of Agriculture, Shihezi University, Shihezi 832000, China.
Shandong Peanut Research Institute, Qingdao 266100, China.
Plants (Basel). 2024 Mar 26;13(7):955. doi: 10.3390/plants13070955.
The () gene family includes zinc finger protein transcription factors that regulate a multitude of physiological and developmental processes in plants. While gene families have been previously determined in various plants, the members and roles of peanut are largely unknown. In this research, on the basis of the genome-wide identification of in three peanut species (, , and ), we investigated the expression profile of the in various tissues and in response to salt and drought stresses and selected for functional characterization. We identified a total of 77 in peanuts, which could be grouped into five subfamilies, with the genes from the same branch of the same subgroup having comparable exon-intron structures. In addition, a significant number of -regulatory elements involved in the regulation of responses to light and hormones and abiotic stresses were found in the promoter region of peanut . Based on the analysis of transcriptome data and qRT-PCR, we identified , , , and as potential genes associated with tolerance to salt and drought. Silencing using virus-induced gene silencing compromised the tolerance of peanut plants to salt and drought stresses. The results of this study provide knowledge on peanut and establish a foundation for future research into their functional roles in peanut development and stress response.
（）基因家族包含锌指蛋白转录因子，这些转录因子调控植物中的多种生理和发育过程。虽然此前已在多种植物中确定了基因家族，但花生（）的成员和作用在很大程度上尚不清楚。在本研究中，基于对三种花生品种（、和）全基因组的鉴定，我们研究了（）在不同组织中的表达谱以及对盐和干旱胁迫的响应，并选择进行功能表征。我们在花生中总共鉴定出77个（），它们可分为五个亚家族，同一亚组同一分支的基因具有相似的外显子 - 内含子结构。此外，在花生（）的启动子区域发现了大量参与光、激素和非生物胁迫响应调控的顺式作用元件。基于转录组数据分析和qRT-PCR，我们鉴定出、、和为与耐盐和耐旱相关的潜在基因。利用病毒诱导基因沉默技术沉默后，花生植株对盐和干旱胁迫的耐受性降低。本研究结果提供了关于花生（）的知识，并为未来研究它们在花生发育和胁迫响应中的功能作用奠定了基础。
