Department of Veterinary Medicine, Guangdong Ocean University, Zhanjiang, Guangdong, P. R. China.
College of Pharmacy, Queen's University, Belfast, Northern Ireland, UK.
J Sep Sci. 2020 Jun;43(12):2301-2310. doi: 10.1002/jssc.201901086. Epub 2020 Apr 20.
The naturally occurring quercetin flavonoid, dihydroquercetin, is widely distributed in plant tissues and has a variety of biological activities. Herein, a magnetic molecularly imprinted solid-phase extraction was tailor made for selective determination of dihydroquercetin in Larix griffithiana using high-performance liquid chromatography. Amino-functionalized core-shell magnetic nanoparticles were prepared and characterized using scanning electron microscopy, transmission electron microscopy, vibrating sample magnetometry, and infrared spectroscopy. The polymer had an average diameter of 250 ± 2.56 nm and exhibited good stability and adsorption for template molecule, which is enriched by hydrogen bonding interaction. Multiple factors for extraction, including loading, washing, elution solvents, and extraction time, were optimized. The limit of detection was 1.23 μg/g. The precision determined at various concentration of dihydroquercetin was less than 4% and the mean recovery was between 74.64 and 101.80%. It has therefore been shown that this protocol can be used as an alternative extraction to quantify dihydroquercetin in L. griffithiana and purify quercetin flavonoid from other complex matrices.
槲皮素类黄酮二氢槲皮素是一种天然存在的物质，广泛分布于植物组织中，具有多种生物活性。本文采用高效液相色谱法，为选择性测定落叶松中的二氢槲皮素，定制了一种基于磁性分子印迹固相萃取的方法。采用扫描电子显微镜、透射电子显微镜、振动样品磁强计和红外光谱对氨基功能化核壳磁性纳米粒子进行了表征。聚合物的平均粒径为 250±2.56nm，表现出良好的模板分子稳定性和吸附性能，这是通过氢键相互作用富集的。优化了萃取的多个因素，包括负载量、洗涤、洗脱溶剂和萃取时间。检测限为 1.23μg/g。在不同浓度的二氢槲皮素测定时，精密度小于 4%，平均回收率在 74.64%至 101.80%之间。因此，该方案可用于替代提取方法，以定量测定落叶松中的二氢槲皮素，并从其他复杂基质中纯化槲皮素类黄酮。
