解析 microRNAs 作为多囊卵巢综合征诊断标志物的作用：系统评价和荟萃分析。

Dissecting the role of micro-RNAs as a diagnostic marker for polycystic ovary syndrome: a systematic review and meta-analysis.

机构信息

Centre for Medical Biotechnology, Maharshi Dayanand University, Haryana, India.

出版信息

Fertil Steril. 2020 Mar;113(3):661-669.e2. doi: 10.1016/j.fertnstert.2019.11.001.

DOI:10.1016/j.fertnstert.2019.11.001

PMID:32192599

Abstract

OBJECTIVE

To investigate the clinical diagnostic value and role of micro-RNAs (miRNAs) in the pathogenesis of polycystic ovary syndrome (PCOS).

DESIGN

Systematic review and meta-analysis.

SETTING

Not applicable.

PATIENT(S): Patients were women of reproductive age with PCOS and controls.

INTERVENTION(S): Summary odds ratio was calculated using a random effects model.

MAIN OUTCOME MEASURE(S): Association of micro-RNAs with PCOS.

METHOD(S): An electronic literature search was conducted using PubMed, Scopus, and Google Scholar databases to identify all relevant studies up to May 2019. A random effects model was used to conduct a meta-analysis. Fold change and P values were used to pool effect size. A funnel plot was used to assess publication bias. Quality score was calculated using the QUADAS scale. Subgroup analysis was based on tissue type. Odds ratios, 95% confidence intervals, and P values were estimated using meta-analysis. Metaregression was performed for correlating covariates with effect size. Area under the curve and receiver operating characteristic analysis was done to assess diagnostic performance accuracy of miRNAs in PCOS.

RESULT(S): Twenty-one studies with a total of 79 miRNAs were included initially. Only three miRNAs (miR-29a-5p, miR-320, miR-93) are reported in more than three studies as of December 2018, so 12 studies were finally included in the quantitative analysis of meta-analysis and 21 studies were involved in the systematic review. The micro-RNAs miR-29a-5p and miR-320 were found to be significantly associated with PCOS. Funnel plot revealed an absence of publication bias for miR-29a-5p and miR-320. Receiver operating characteristic analysis with an area under the curve value of 0.95 proved miR-29a-5p to be the better diagnostic marker of PCOS.

CONCLUSION(S): Aberrant expression of various miRNAs plays an important role in PCOS pathogenesis. Micro-RNAs hold potential diagnostic value for PCOS. These findings may offer new insights for PCOS pathogenesis research.

PROSPERO REGISTRATION NUMBER

CRD42018106198.

摘要

目的

探讨微小 RNA（miRNAs）在多囊卵巢综合征（PCOS）发病机制中的临床诊断价值和作用。

设计

系统评价和荟萃分析。

环境

不适用。

患者

患有 PCOS 的育龄期妇女和对照组患者。

干预措施

使用随机效应模型计算汇总优势比。

主要观察指标

miRNAs 与 PCOS 的相关性。

方法

通过电子文献检索 PubMed、Scopus 和 Google Scholar 数据库，检索截至 2019 年 5 月所有相关研究。采用随机效应模型进行荟萃分析。使用 fold change 和 P 值来合并效应量。采用漏斗图评估发表偏倚。使用 QUADAS 量表计算质量评分。根据组织类型进行亚组分析。使用荟萃分析估计比值比、95%置信区间和 P 值。进行 metaregression 以分析协变量与效应量的相关性。采用曲线下面积和受试者工作特征分析评估 miRNAs 在 PCOS 中的诊断性能准确性。

结果

最初共纳入 21 项研究，共计 79 个 miRNAs。截至 2018 年 12 月，仅有 3 个 miRNAs（miR-29a-5p、miR-320、miR-93）有超过 3 项研究报道，因此最终有 12 项研究纳入荟萃分析的定量分析，21 项研究纳入系统评价。miR-29a-5p 和 miR-320 与 PCOS 显著相关。漏斗图显示 miR-29a-5p 和 miR-320 不存在发表偏倚。受试者工作特征分析的曲线下面积值为 0.95，表明 miR-29a-5p 是诊断 PCOS 的更好标志物。