Teixeira Amanda Escobar, Rocha-Gomes Arthur, Pereira Dos Santos Tatiele, Amaral Bianca Lara Silva, da Silva Alexandre Alves, Malagutti Andrea Renata, Leite Fernando Roberto Figueiredo, Stuckert-Seixas Sergio Ricardo, Riul Tania Regina
Nutrition Department, Experimental Nutrition Laboratory (LabNutrex), Universidade Federal dos Vales do Jequitinhonha e Mucuri, Highway MGT 367 - Km 583, Campus JK, n° 5000, Diamantina, 39100000 MG, Brazil; Pharmacy Department, Pharmacology Laboratory, Universidade Federal dos Vales do Jequitinhonha e Mucuri, Highway MGT 367 - Km 583, Campus JK, n° 5000, Diamantina, 39100000 MG, Brazil.
Nutrition Department, Experimental Nutrition Laboratory (LabNutrex), Universidade Federal dos Vales do Jequitinhonha e Mucuri, Highway MGT 367 - Km 583, Campus JK, n° 5000, Diamantina, 39100000 MG, Brazil.
Physiol Behav. 2020 Jun 1;220:112874. doi: 10.1016/j.physbeh.2020.112874. Epub 2020 Mar 16.
To evaluate the nutritional status and behavior of animals fed a cafeteria diet from the onset of lactation after the addition of risperidone.
During the lactation period, 14 litters of Wistar rats (dam + 8 pups) were fed one of two diets: control (CTRL; n = 7) or cafeteria (CAF; n = 7). After weaning, the males were placed in individual cages, receiving the same diet as offered to their respective dams. Food and caloric intake, body weight, feed and energy efficiency, and adipose tissue weight were evaluated in the male offspring. In adulthood, they were assigned to receive treatment with saline (CTRL-S, CAF-S) or risperidone (CTRL-R, CAF-R) (n = 21 in each group). They then underwent behavioral testing, which included the elevated plus maze, open field, object recognition, and social interaction tests. Variance analysis (ANOVA) was used, followed by Newman-Keuls when p-values were < 0.05.
The CAF group exhibited higher caloric intake, weight gain, feed efficiency, and adipose tissue than the CTRL group. The animals in the CAF group exhibited oxidative stress characteristics in the hippocampus, which may have compromised the function of this structure and promoted behavioral changes. The CAF-S group exhibited anxiety, as indicated by the greater number of entrances and time spent in the center of the open field. They also showed greater locomotion through a greater number of quadrants traveled. CAF-S animals also demonstrated memory impairments, assessed using the object recognition test, and decreased social interaction. The CAF-R group demonstrated anxiety and decreased locomotion in the open field. There was a decrease in their interaction with both objects in the object recognition test. The CAF-R group obtained greater sociability in the social interaction test. Such effects may be associated with changes in the serotonergic system of these animals.
Risperidone administered to animals on a cafeteria diet led to a greater reduction in locomotion, had an anxiogenic effect, caused impaired memory, and improved social interaction.
评估从哺乳期开始就喂食自助餐式饮食并添加利培酮的动物的营养状况和行为。
在哺乳期，14窝Wistar大鼠（母鼠 + 8只幼崽）被喂食两种饮食之一：对照组（CTRL；n = 7）或自助餐组（CAF；n = 7）。断奶后，雄性幼崽被放置在单独的笼子里，接受与各自母鼠相同的饮食。对雄性后代的食物和热量摄入、体重、饲料和能量效率以及脂肪组织重量进行评估。成年后，它们被分配接受生理盐水（CTRL-S，CAF-S）或利培酮（CTRL-R，CAF-R）治疗（每组n = 21）。然后对它们进行行为测试，包括高架十字迷宫、旷场试验、物体识别和社交互动测试。使用方差分析（ANOVA），当p值 < 0.05时，接着进行纽曼-丘尔斯检验。
CAF组的热量摄入、体重增加、饲料效率和脂肪组织均高于CTRL组。CAF组动物的海马体表现出氧化应激特征，这可能损害了该结构的功能并促进了行为变化。CAF-S组表现出焦虑，表现为进入旷场中央的次数更多和在中央花费的时间更长。它们还通过走过更多象限表现出更大的活动量。使用物体识别测试评估，CAF-S动物还表现出记忆障碍和社交互动减少。CAF-R组在旷场试验中表现出焦虑和活动量减少。在物体识别测试中，它们与两个物体的互动都减少了。CAF-R组在社交互动测试中表现出更高的社交能力。这些影响可能与这些动物血清素系统的变化有关。
给食用自助餐式饮食的动物施用利培酮会导致活动量更大幅度的减少，产生焦虑作用，导致记忆受损，并改善社交互动。
