An EPR Strategy for Bio-responsive Fluorescence Guided Surgery with Simulation of the Benefit for Imaging.

A successful matching of a PEG group size with the EPR effect for an responsive NIR-fluorophore conjugate has been accomplished which allows two distinct tumor imaging periods, the first being the switch on during the initial tumor uptake via enhanced permeability into the ROI (as background is suppressed) and a second, later, due to enhanced retention within the tumor. : Software simulation (https://mihaitodor.github.io/particle_simulation/index.html), synthetic chemistry, with and imaging have been synergistically employed to identify an optimal PEG conjugate of a bio-responsive NIR-AZA fluorophore for tumor imaging. : A bio-responsive NIR-AZA fluorophore conjugated to a 10 kDa PEG group has shown excellent imaging performance with sustained high tumor to background ratios and peak tumor emission within 24 h. Analysis of fluorescence profiles over 7 days has provided evidence for the EPR effect playing a positive role. : Preclinical results show that exploiting the EPR effect by utilizing an optimized PEG substituent on a bio-responsive fluorophore may offer a means for intraoperative tumor margin delineation. The responsive nature of the fluorophore makes tumor imaging achievable without waiting for clearance from normal tissue.