Caulfield Cathal, Wu Dan, Garre Massimiliano, O'Shea Donal F
Department of Chemistry, RCSI 123 St. Stephen's Green Dublin 2 Ireland
RSC Adv. 2023 May 16;13(22):14963-14973. doi: 10.1039/d2ra07942a. eCollection 2023 May 15.
Three bis(anilino)-substituted NIR-AZA fluorophores have been designed, synthesized and tested to bridge the availability gap of molecular fluorophores for live-cell microscopy imaging in the 800-850 nm spectral range. The concise synthetic route allows for the later stage introduction of three tailored peripheral substituents which guides the sub-cellular localization and imaging. Live-cell fluorescence imaging of lipid droplets, plasma membrane and cytosolic vacuoles was successfully achieved. Photophysical and internal charge transfer (ICT) properties of each fluorophore were examined through solvent studies and analyte responses.
设计、合成并测试了三种双（苯胺基）取代的近红外氮杂荧光团，以填补800-850nm光谱范围内用于活细胞显微镜成像的分子荧光团可用性的空白。简洁的合成路线允许在后期引入三个定制的外围取代基，以指导亚细胞定位和成像。成功实现了脂滴、质膜和胞质液泡的活细胞荧光成像。通过溶剂研究和分析物响应研究了每个荧光团的光物理和内电荷转移（ICT）性质。
