Akbari Afjani Javad, Soltani Najafabadi Masood, Gholi Mirfakhraei Reza
Department of Biotechnology, Faculty of Agriculture, Tarbiat Modarres University, Tehran, Iran.
Seed and Plant Improvement Institute (SPII), Agricultural Research, Education and Extension (AREEO), Shahid Fahmideh BLVRD, Seed and Plant Improvement Institute Campus, Karaj, Iran.
Iran J Biotechnol. 2019 Sep 1;17(3):e1870. doi: 10.29252/ijb.1870. eCollection 2019 Sep.
In plant breeding program to produce hybrid varieties, pair of male sterile and restorer fertility lines are required. Differentiation of lines possessing restorer fertility allele from the lines lacking it remove the need for the progeny test, and thus reducing the time and the cost in the hybrid production procedure. Canola breeding program in Iran has concentrated toward production of domestic hybrid varieties, however, it suffers from lack of molecular information in restore fertility status of lines, and therefore it needs time and tedious activities.
To design gene-based markers for distinguishing R-, A- lines and hybrids in sunflower breeding programs.
Aligning sequences of locus responsible for male sterility and that of male fertility resulted in finding differences in the loci, which used to define two set of suitable primer pairs. Genomic DNA from 25 R-lines (23 inbred lines and two commercial lines), 9 A-lines (7 inbred lines and two commercial lines), one B-line and two commercial hybrids were extracted and used in PCR as template.
Using one-primer pairs, a band of nearly 1500 bp was amplified in restorer lines but not in A-, B- lines. Another primer pair used to distinguish hybrids (heterozygout) from restorer lines. Results of the report is predicted to be used in canola breeding for hybrid production.
Although the molecular bases for the male sterility and fertility restoration in rapeseed is not published, taking advantages of gene-based markers, make rapeseed breeding program more efficient regarding time and costs.
在培育杂交品种的植物育种计划中，需要雄性不育系和恢复系这一对品系。区分具有恢复育性等位基因的品系和缺乏该等位基因的品系，无需进行后代测试，从而减少了杂交生产过程中的时间和成本。伊朗的油菜育种计划一直致力于培育国内杂交品种，然而，在品系恢复育性状态方面缺乏分子信息，因此需要耗费时间且活动繁琐。
设计基于基因的标记，用于在向日葵育种计划中区分R系、A系和杂交种。
对负责雄性不育的基因座序列和雄性育性的基因座序列进行比对，发现了基因座上的差异，据此定义了两组合适的引物对。提取了来自25个R系（23个自交系和2个商业品系）、9个A系（7个自交系和2个商业品系）、1个B系和2个商业杂交种的基因组DNA，并用作PCR模板。
使用一对引物时，在恢复系中扩增出一条近1500 bp的条带，而在A系和B系中未扩增出。另一对引物用于区分杂交种（杂合子）和恢复系。预计该报告结果将用于油菜杂交种生产育种。
尽管油菜中雄性不育和育性恢复的分子基础尚未公布，但利用基于基因的标记，可使油菜育种计划在时间和成本方面更高效。
