Fan Jing-Ran, Kuang Yi, Dong Ze-Yuan, Yi Yang, Zhou Yan-Xia, Li Bin, Qiao Xue, Ye Min
State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, 38 Xueyuan Road, Beijing 100191, People's Republic of China.
School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100029, People's Republic of China.
J Nat Prod. 2020 Apr 24;83(4):814-824. doi: 10.1021/acs.jnatprod.9b00262. Epub 2020 Mar 20.
(liquorice) is a well-known medicinal plant. Its roots and rhizomes are used as the popular Chinese herbal medicine Gan-Cao. An ethanol extract of the aerial parts of showed antidiabetic effects on db/db mice. It decreased the blood glucose level by 30.3% and increased the serum insulin level by 41.8% compared to the control group. Eighty-six phenolic compounds (-) were obtained from the aerial parts, including the new prenylated isoflavanones (-), isoflavans (-), and a 2-phenylbenzofuran (). The structures were identified by NMR and HRESIMS data analyses, and the absolute configurations were established by comparing the calculated and experimental ECD spectroscopic data. Compounds , , and inhibited PTP1B with IC values of 5.9, 6.7, and 5.3 μM, respectively. Compound and the known compounds glycycoumarin () and glyurallin A () inhibited α-glucosidase with IC values of 20.1, 0.1, and 0.3 μM, respectively. Compound at 10 μM increased the glucose uptake rate to 95% in an insulin resistance HepG2 cell model ( < 0.01).
甘草是一种著名的药用植物。其根和根茎被用作 popular Chinese herbal medicine Gan-Cao。甘草地上部分的乙醇提取物对 db/db 小鼠显示出抗糖尿病作用。与对照组相比，其血糖水平降低了 30.3%，血清胰岛素水平升高了 41.8%。从地上部分获得了 86 种酚类化合物（-），包括新的异戊烯基化异黄酮（-）、异黄烷（-）和一种 2-苯基苯并呋喃（）。通过 NMR 和 HRESIMS 数据分析鉴定了结构，并通过比较计算和实验 ECD 光谱数据确定了绝对构型。化合物、和分别以 5.9、6.7 和 5.3 μM 的 IC 值抑制 PTP1B。化合物和已知化合物光甘草定（）和光甘草灵 A（）分别以 20.1、0.1 和 0.3 μM 的 IC 值抑制 α-葡萄糖苷酶。在胰岛素抵抗的 HepG2 细胞模型中，10 μM 的化合物使葡萄糖摄取率提高到 95%（<0.01）。
