Kingsmore S F, Crockard A D, Fay A C, McNeill T A, Roberts S D, Thompson J M
Department of Microbiology and Immunobiology, Queen's University of Belfast, Royal Victoria Hospital, Northern Ireland.
Diagn Clin Immunol. 1988;5(6):289-96.
Several flow cytometric methods for the measurement of circulating immune complexes (CIC) have recently become available. We report a Raji cell flow cytometric assay (FCMA) that uses aggregated human globulin (AHG) as primary calibrator. Technical advantages of the Raji cell flow cytometric assay are discussed, and its clinical usefulness is evaluated in a method comparison study with the widely used Raji cell immunoradiometric assay. FCMA is more precise and has greater analytic sensitivity for AHG. Diagnostic sensitivity by the flow cytometric method is superior in systemic lupus erythematosus (SLE), rheumatoid arthritis, and vasculitis patients: however, diagnostic specificity is similar for both assays, but the reference interval of FCMA is narrower. Significant correlations were found between CIC levels obtained with both methods in SLE, rheumatoid arthritis, and vasculitis patients and in longitudinal studies of two patients with cerebral SLE. The Raji cell FCMA is recommended for measurement of CIC levels to clinical laboratories with access to a flow cytometer.
最近出现了几种用于测量循环免疫复合物（CIC）的流式细胞术方法。我们报告了一种使用聚合人球蛋白（AHG）作为一级校准物的拉吉细胞流式细胞术测定法（FCMA）。讨论了拉吉细胞流式细胞术测定法的技术优势，并在与广泛使用的拉吉细胞免疫放射测定法的方法比较研究中评估了其临床实用性。FCMA对AHG更精确且具有更高的分析灵敏度。流式细胞术方法在系统性红斑狼疮（SLE）、类风湿性关节炎和血管炎患者中的诊断敏感性更高：然而，两种测定法的诊断特异性相似，但FCMA的参考区间更窄。在SLE、类风湿性关节炎和血管炎患者以及两名脑型SLE患者的纵向研究中，两种方法获得的CIC水平之间存在显著相关性。建议有条件使用流式细胞仪的临床实验室采用拉吉细胞FCMA来测量CIC水平。
