Crockard A D, Thompson J M, Finch M B, McNeill T A, Bell A L, Roberts S D
Department of Microbiology and Immunobiology, Royal Victoria Hospital, Queen's University of Belfast, Northern Ireland.
Rheumatol Int. 1991;11(4-5):169-74. doi: 10.1007/BF00332556.
The immunoglobulin (Ig) heavy chain isotype composition of intra-articular and circulating immune complexes (ICs) were determined by a Raji cell flow cytometric assay in paired serum and synovial fluid samples from 15 patients with rheumatoid arthritis (RA) and 15 patients with other articular diseases (osteoarthritis, ankylosing spondylitis, gout, psoriatic arthritis, Reiter's disease). ICs were most prevalent in synovial fluid samples of patients with RA but were infrequently detected in serum and synovial fluid samples from the non-RA patients. ICs in patients with RA were heterogeneous both in the prevalence of Ig subclasses identified and in the distribution of the respective Ig isotypes within the complexes. Furthermore, differences were observed in the Ig isotype composition of ICs in paired serum and synovial fluid samples indicating that circulating ICs may not always arise simply by spill-over from articular sites. The possible mechanisms for IC formation in RA are discussed with reference to four patients who displayed features of extra-articular disease.
通过拉吉细胞流式细胞术检测了15例类风湿关节炎（RA）患者和15例其他关节疾病（骨关节炎、强直性脊柱炎、痛风、银屑病关节炎、赖特综合征）患者配对的血清和滑液样本中关节内和循环免疫复合物（ICs）的免疫球蛋白（Ig）重链同种型组成。ICs在RA患者的滑液样本中最为常见，但在非RA患者的血清和滑液样本中很少检测到。RA患者的ICs在所鉴定的Ig亚类患病率以及复合物内各Ig同种型的分布方面均具有异质性。此外，在配对的血清和滑液样本中观察到ICs的Ig同种型组成存在差异，这表明循环ICs可能并非总是简单地由关节部位溢出产生。参考4例表现出关节外疾病特征的患者，讨论了RA中IC形成的可能机制。
