采用间接粒细胞吞噬试验通过流式细胞术测定循环免疫复合物。
Flow cytometric determination of circulating immune complexes with the indirect granulocyte phagocytosis test.
作者信息
Terstappen L W, de Grooth B G, Nolten G M, ten Napel C H, van Berkel W, Greve J
出版信息
Cytometry. 1985 Jul;6(4):316-20. doi: 10.1002/cyto.990060407.
A method for the determination of circulating immune complexes (CIC) was adapted for flow cytometric analysis. Human granulocytes were used to phagocytose IgG-bearing CIC of serum from systemic lupus erythematosus (SLE) patients. A method for labeling the phagocytosed CIC with FITC-conjugated anti-human IgG was developed where the granulocytes remain in suspension during fixation and labeling. The fluorescence per cell, measured with a flow cytometer, is a measure of the total amount of the phagocytosed IgG. The results indicate that a rapid and quantitative method for the detection and measurement of phagocytosed CIC is possible using the flow cytometer.
一种用于测定循环免疫复合物（CIC）的方法被应用于流式细胞术分析。使用人粒细胞吞噬系统性红斑狼疮（SLE）患者血清中携带IgG的CIC。开发了一种用异硫氰酸荧光素（FITC）偶联的抗人IgG标记吞噬的CIC的方法，其中粒细胞在固定和标记过程中保持悬浮状态。用流式细胞仪测量的每个细胞的荧光是吞噬的IgG总量的一种度量。结果表明，使用流式细胞仪可以实现一种快速、定量检测和测量吞噬的CIC的方法。
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