Influence of in vitro susceptibility testing conditions on the anti-candidal activity of LY121019.

LY121019 is a new antifungal antimicrobic that is structurally similar to the lipopeptide agents echinocandin B and aculeacin A. Because of the importance of in vitro test conditions on the activity of other antifungal agents, we studied the effects of inoculum size, time and temperature of incubation, pH, and medium composition on the in vitro activity of LY121019 against Candida albicans, Candida tropicalis, and Candida parapsilosis. LY121019 was highly active against Candida albicans and Candida tropicalis and inactive against Candida parapsilosis. The in vitro activity of LY121019 is marked by a paradoxical dose-response with isolates of Candida albicans and Candida tropicalis and is influenced by choice of inoculum size, time and temperature of incubation, medium composition, and pH. We recommend the use of an inoculum size of less than 10(5) organisms/ml, a defined medium buffered to a pH of 7.0, and incubation at 30 degrees C for 24 hr for future in vitro studies of LY121019.