Compton M R, Hillery S J, Kerr S J, Seale J P
Department of Pharmacology, University of Sydney, N.S.W., Australia.
Eur J Pharmacol. 1988 Dec 6;158(1-2):129-34. doi: 10.1016/0014-2999(88)90261-0.
Groups of guinea-pigs were sensitized to ovalbumin, using different regimens to induce either IgG-like antibodies or IgE-like and IgG-like antibodies and the responsiveness to histamine and carbachol in vitro of tracheal and lung parenchymal strips was determined. EC50 values for histamine and carbachol in both tracheal and lung parenchymal strips were not significantly different for either group of sensitized guinea-pigs or their respective controls. The maximal tension developed in parenchymal strips was not significantly different for either sensitized or control groups. For tracheal strips, maximal tension was not significantly different in each group except in animals with IgG-like antibodies, which developed less maximal tension with histamine than controls. The absence of any increase in maximal tension or any change in EC50 to histamine or to carbachol in tracheal or parenchymal strips from immunized guinea-pigs indicates that immunological sensitization does not increase the responsiveness of smooth muscle to these contractile agonists.
将豚鼠分组，用不同方案使其对卵清蛋白致敏，以诱导产生IgG样抗体或IgE样及IgG样抗体，然后测定气管和肺实质条带对组胺和卡巴胆碱的体外反应性。致敏豚鼠组及其各自对照组的气管和肺实质条带中组胺和卡巴胆碱的半数有效浓度（EC50）值无显著差异。实质条带产生的最大张力在致敏组和对照组中无显著差异。对于气管条带，除了产生IgG样抗体的动物外，每组的最大张力无显著差异，这些动物用组胺产生的最大张力低于对照组。免疫豚鼠的气管或实质条带中最大张力没有增加，对组胺或卡巴胆碱的EC50也没有变化，这表明免疫致敏不会增加平滑肌对这些收缩激动剂的反应性。
