Department of Pharmacology, Amrita School of Pharmacy, Amrita Vishwa Vidyapeetham , Kochi, India.
Amrita Centre for Nano Sciences and Molecular Medicine, Amrita Vishwa Vidyapeetham , Kochi, India.
Clin Exp Hypertens. 2020 Oct 2;42(7):581-589. doi: 10.1080/10641963.2020.1739699. Epub 2020 Mar 21.
The present work aimed to study the effect of aqueous extract of large cardamom (AELC) to prevent vascular remodeling and oxidative stress in N-Nitro-L-arginine methyl ester (L-NAME)-induced hypertension.
Male Wistar rats were administered with L-NAME 40 mg/kg/day for 28 days by oral gavage. The treatments included captopril (20 mg/kg/day) or AELC (100, 200 and 400 mg/kg/day) along with L-NAME administration.
L-NAME treated rats showed high systolic, diastolic and mean arterial pressure, decreased nitric oxide level, increased level of malondialdehyde in plasma, heart, aorta and kidney, hypertrophy of the vascular wall and reduced vascular response to acetylcholine in phenylephrine-precontracted aorta. Treatment with AELC markedly reduced the blood pressure, restored the nitric oxide level, reduced the malondialdehyde level, alleviated the hypertrophy in L-NAME-induced hypertensive rats. Additionally, it also improved the vascular response to acetylcholine in phenylephrine pre-contracted aorta.
In conclusion, our results demonstrate the preventive effect of AELC in L-NAME-induced hypertensive model, which is possibly related to antioxidant activities and restoration of nitric oxide level.
本研究旨在探讨大腹皮水提物（AELC）对 N-硝基-L-精氨酸甲酯（L-NAME）诱导的高血压中血管重塑和氧化应激的预防作用。
雄性 Wistar 大鼠通过灌胃给予 L-NAME 40 mg/kg/天，共 28 天。治疗组包括卡托普利（20 mg/kg/天）或 AELC（100、200 和 400 mg/kg/天）与 L-NAME 同时给药。
L-NAME 处理的大鼠表现出较高的收缩压、舒张压和平均动脉压，一氧化氮水平降低，血浆、心脏、主动脉和肾脏中丙二醛水平升高，血管壁肥厚，苯肾上腺素预收缩的主动脉对乙酰胆碱的血管反应降低。AELC 治疗显著降低了血压，恢复了一氧化氮水平，降低了丙二醛水平，减轻了 L-NAME 诱导的高血压大鼠的肥厚。此外，它还改善了苯肾上腺素预收缩的主动脉对乙酰胆碱的血管反应。
总之，我们的结果表明 AELC 对 L-NAME 诱导的高血压模型具有预防作用，这可能与抗氧化活性和一氧化氮水平的恢复有关。
