The Key Laboratory of Leather Chemistry and Engineering of Ministry of Education, College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China.
Textile Institute, College of Biomass Science and Engineering, Sichuan University, Chengdu 610065, China.
ACS Appl Mater Interfaces. 2020 Apr 8;12(14):16125-16136. doi: 10.1021/acsami.0c02143. Epub 2020 Mar 27.
Ocular dryness and contact lens(CL)-related microbial keratitis (MK) are two major risks of wearing CLs. The development of multifunctional surface coating for CLs with excellent hydrating and antimicrobial properties is a practical strategy to improve the comfort of CL wearers and to prevent corneal infection. Here, we develop zwitterionic and antimicrobial metal-phenolic networks (MPNs) based on the coordination of copper ions (Cu) and the poly(carboxylbetaine--dopamine methacrylamide) copolymer (PCBDA), which can be easily one-step prepared onto CLs due to the near-universal adherent properties of catechol groups. The zwitterionic and antifouling carboxybetaine (CB) groups of the Cu-PCBDA coating can significantly increase the wettability of CLs and reduce their protein adsorptions, resulting in a lens surface that is more water retentive and with lower protein binding to prevent tear film evaporation and eye dryness. In addition, since the immobilized copper ions in the MPNs impart them with ion-mediated antimicrobial activity, the Cu-PCBDA coating exhibits a strong and broad-spectrum antimicrobial activity against MK related pathogenic microbes, including bacteria, such as , , and , and fungi, such as . Compared with a pristine CL, the Cu-PCBDA-coated CL effectively inhibited biofilm formation even after daily exposure to the above microbial environment for 14 days. Notably, the Cu-PCBDA coating developed in this study is not only biocompatible with 100% cell viability following direct contact with human corneal epithelial cells (HCECs) for 48 h but also maintains the optical clarity of the native CLs. Thus, the Cu-PCBDA coating has a great application potential for the development of a multifunctional surface coating for CLs for increased CL comfort and prevention of MK.
眼干燥和接触镜（CL）相关微生物角膜炎（MK）是佩戴 CL 的两个主要风险。开发具有优异保湿和抗菌性能的多功能 CL 表面涂层是提高 CL 佩戴者舒适度和预防角膜感染的实用策略。在这里，我们开发了基于铜离子（Cu）和聚（羧基甜菜碱-多巴胺甲基丙烯酰胺）共聚物（PCBDA）配位的两性离子和抗菌金属-酚网络（MPN），由于儿茶酚基团的普遍附着特性，可轻松地将其一步制备到 CL 上。Cu-PCBDA 涂层的两性离子和抗污羧基甜菜碱（CB）基团可显著提高 CL 的润湿性并减少其蛋白质吸附，从而使镜片表面具有更高的保水性和更低的蛋白质结合，以防止泪膜蒸发和眼睛干燥。此外，由于 MPN 中固定的铜离子赋予其离子介导的抗菌活性，因此 Cu-PCBDA 涂层对与 MK 相关的致病微生物表现出强大且广谱的抗菌活性，包括细菌，如 ， 和 ，和真菌，如 。与原始 CL 相比，即使在每天暴露于上述微生物环境 14 天后，Cu-PCBDA 涂层也能有效抑制生物膜形成。值得注意的是，与直接接触人角膜上皮细胞（HCEC）48 h 后 100%细胞活力的研究中开发的 Cu-PCBDA 涂层不仅具有生物相容性，而且还保持了天然 CL 的光学清晰度。因此，Cu-PCBDA 涂层在开发用于增加 CL 舒适度和预防 MK 的 CL 多功能表面涂层方面具有很大的应用潜力。
