Department of Microbiology and Immunology, University of California, San Francisco, CA, USA.
Section of Microbiology, University of Copenhagen, Copenhagen, Denmark.
Nat Methods. 2020 May;17(5):471-479. doi: 10.1038/s41592-020-0771-6. Epub 2020 Mar 16.
Clustered, regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) genes, a diverse family of prokaryotic adaptive immune systems, have emerged as a biotechnological tool and therapeutic. The discovery of protein inhibitors of CRISPR-Cas systems, called anti-CRISPR (Acr) proteins, enables the development of more controllable and precise CRISPR-Cas tools. Here we discuss applications of Acr proteins for post-translational control of CRISPR-Cas systems in prokaryotic and mammalian cells, organisms and ecosystems.
成簇、规律间隔短回文重复序列 (CRISPR) 和 CRISPR 相关 (Cas) 基因是一类多样化的原核适应性免疫系统，已成为生物技术工具和治疗方法。CRISPR-Cas 系统蛋白抑制剂的发现，即抗 CRISPR（Acr）蛋白，使得更可控和精确的 CRISPR-Cas 工具得以开发。在这里，我们讨论了 Acr 蛋白在原核细胞和哺乳动物细胞、生物和生态系统中转录后调控 CRISPR-Cas 系统的应用。
